ProtParam
User-provided sequence:
10 20 30 40 50
MTIAPITGTI KRRVIMDIVL GFSLGGVMAS YWWWGFHMDK INKREKFYAE LAERKKQEN
References and
documentation are available.
Number of amino acids: 59
Molecular weight: 6963.22
Theoretical pI: 9.77
Total number of negatively charged residues (Asp + Glu): 6
Total number of positively charged residues (Arg + Lys): 10
Atomic composition:
Carbon C 319
Hydrogen H 497
Nitrogen N 85
Oxygen O 82
Sulfur S 4
Formula: C
319H
497N
85O
82S
4
Total number of atoms: 987
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 19480
Abs 0.1% (=1 g/l) 2.798
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.23
This classifies the protein as unstable.
Aliphatic index: 81.02
Grand average of hydropathicity (GRAVY): -0.246