ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTIDDSNRLL MDVDQFDFLD DGTAQLSNNK TDEEEQLYKR DPVSGAILVP MTVNDQPIEK 

        70         80         90        100        110        120 
NGDKMPLKFK LGPLSYQNMA FITAKDKYKL YPVRIPRLDT SKEFSAYVSG LFEIYRDLGD 

       130        140        150        160        170        180 
DRVFNVPTIG VVNSNFAKEH NATVNLAMEA ILNELEVFIG RVKDQDGRVN RFYELEESLT 

       190        200        210        220        230        240 
VLNCLRTMYF ILDGQDVEEN RSEFIESLLN WINRSDGEPD EEYIEQVFSV KDSTAGKKVF 

       250        260        270        280        290        300 
ETQYFWKLLN QLVLRGLLSQ AIGCIERSDL LPYLSDTCAV SFDAVSDSIE LLKQYPKDSS 

       310        320        330        340        350        360 
STFREWKNLV LKLSQAFGSS ATDISGELRD YIEDFLLVIG GNQRKILQYS RTWYESFCGF 

       370        380        390        400        410        420 
LLYYIPSLEL SAEYLQMSLE ANVVDITNDW EQPCVDIISG KIHSILPVME SLDSCTAAFT 

       430        440        450        460        470        480 
AMICEAKGLI ENIFEGEKNS DDYSNEDNEM LEDLFSYRNG MASYMLNSFA FELCSLGDKE 

       490        500        510        520        530        540 
LWPVAIGLIA LSATGTRSAK KMVIAELLPH YPFVTNDDIE WMLSICVEWR LPEIAKEIYT 

       550        560        570        580        590        600 
TLGNQMLSAH NIIESIANFS RAGKYELVKS YSWLLFEASC MEGQKLDDPV LNAIVSKNSP 

       610        620        630        640        650        660 
AEDDVIIPQD ILDCVVTNSM RQTLAPYAVL SQFYELRDRE DWGQALRLLL LLIEFPYLPK 

       670        680        690        700        710        720 
HYLVLLVAKF LYPIFLLDDK KLMDEDSVAT VIEVIETKWD DADEKSSNLY ETIIEADKSL 

       730        740 
PSSMATLLKN LRKKLNFKLC QAFM 

References and documentation are available.

Number of amino acids: 744

Molecular weight: 84897.84

Theoretical pI: 4.50

Amino acid composition: 
Ala (A)  44	  5.9%
Arg (R)  27	  3.6%
Asn (N)  38	  5.1%
Asp (D)  58	  7.8%
Cys (C)  12	  1.6%
Gln (Q)  24	  3.2%
Glu (E)  61	  8.2%
Gly (G)  30	  4.0%
His (H)   5	  0.7%
Ile (I)  49	  6.6%
Leu (L)  94	 12.6%
Lys (K)  43	  5.8%
Met (M)  21	  2.8%
Phe (F)  35	  4.7%
Pro (P)  26	  3.5%
Ser (S)  61	  8.2%
Thr (T)  30	  4.0%
Trp (W)  11	  1.5%
Tyr (Y)  31	  4.2%
Val (V)  44	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 119
Total number of positively charged residues (Arg + Lys): 70

Atomic composition:

Carbon      C	      3818
Hydrogen    H	      5943
Nitrogen    N	       951
Oxygen      O	      1167
Sulfur      S	        33

Formula: C3818H5943N951O1167S33
Total number of atoms: 11912

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   107440
Abs 0.1% (=1 g/l)   1.266, assuming all pairs of Cys residues form cystines


Ext. coefficient   106690
Abs 0.1% (=1 g/l)   1.257, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.69
This classifies the protein as unstable.



Aliphatic index: 98.02

Grand average of hydropathicity (GRAVY): -0.138