ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTIDGTGQSK EALQDERLNT GSDKVYQNYM MPALELYDAK VSINHWQLRD CIKPGSMNQS 

        70         80         90        100        110        120 
KLYYIYDHSI RVLDTDSSVL RSPVRRHNSI QPSNSGKNST EKTSTKGSRT TGSYISKNLH 

       130        140        150        160        170        180 
VPSEKLVEFN FKPRCFTELN GLTVCGGLIG SDDKGFPSNW NRLAQDANIS LPPPSQPINI 

       190        200        210        220        230        240 
SKNISFPINS HYSNPNIWKG IVEFYNQETD TMMTFTLGQF INNCVTLYDR ASMQFDLFAC 

       250        260        270        280        290        300 
NNDGHLYQCD VSNRDVTLVK RYADLKFPLN NASLSHDGQT MVVSGDSNKF AVYNQNELTN 

       310        320        330        340        350        360 
QFSLHYDNHP SWGSSVNRVR RIPRFALPDE SEYIENIYEA PNSDHGFYNS FSENDLQFAT 

       370        380        390        400        410        420 
VFQNGTCAIY DIRNMATPMA EISSTRPHSH NGAFRVCRFS YGLDDLLFIS EHQGRVHVVD 

       430        440        450        460        470        480 
TRNYVNHQVI VIPDKVNMEY INERKHNTNH NFTTNNNNEN ESNDSKNELQ GADYRSLSRR 

       490        500        510        520        530        540 
RFSLPSMPNV TTEPWITMAQ RIPKKYLEPQ ILPFPKVMDK ISNESVLFST KGSSSSDVAH 

       550        560        570        580        590        600 
PYKRRCSFRV RRVSTSAPTA DYSNNNVNAS LGTPAADSIA TSSSNSAPQN LIDPLILSHQ 

       610        620        630        640        650        660 
QASNDVFEDD EYYEAYNDVH STYRVSSDYH GVSARAFESF LRPPSTPDLP SDDDNFAANS 

       670        680        690        700        710        720 
RNNRGTSNFL RRPVITTQES NEFSEENNIS GIDWVEDRNG SSLIIGTDYG IMRWNINSWA 

       730 
RRSFSSYDLC 

References and documentation are available.

Number of amino acids: 730

Molecular weight: 82799.36

Theoretical pI: 5.91

Amino acid composition: 
Ala (A)  34	  4.7%
Arg (R)  44	  6.0%
Asn (N)  74	 10.1%
Asp (D)  49	  6.7%
Cys (C)  10	  1.4%
Gln (Q)  26	  3.6%
Glu (E)  34	  4.7%
Gly (G)  32	  4.4%
His (H)  21	  2.9%
Ile (I)  40	  5.5%
Leu (L)  45	  6.2%
Lys (K)  26	  3.6%
Met (M)  15	  2.1%
Phe (F)  33	  4.5%
Pro (P)  38	  5.2%
Ser (S)  88	 12.1%
Thr (T)  41	  5.6%
Trp (W)   8	  1.1%
Tyr (Y)  32	  4.4%
Val (V)  40	  5.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 83
Total number of positively charged residues (Arg + Lys): 70

Atomic composition:

Carbon      C	      3610
Hydrogen    H	      5528
Nitrogen    N	      1038
Oxygen      O	      1158
Sulfur      S	        25

Formula: C3610H5528N1038O1158S25
Total number of atoms: 11359

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    92305
Abs 0.1% (=1 g/l)   1.115, assuming all pairs of Cys residues form cystines


Ext. coefficient    91680
Abs 0.1% (=1 g/l)   1.107, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.13
This classifies the protein as unstable.



Aliphatic index: 65.96

Grand average of hydropathicity (GRAVY): -0.688