ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTIHQLSPES QWKIVSSSFI YGPVAAVREL LDNSIDSGAK KVFIDVDSTT GGCEYISVKD 

        70         80         90        100        110        120 
DGSGVDIIDR PSMCLEYTTS KMSSLGDISI LTTLGFRGEA LFLLSNLCNQ KGSMQVETKT 

       130        140        150        160        170        180 
ADDVIGEKWL VDSKGGITNG KRYKVSCPVG TTVILRKLLG GLRARYLEIS SRPRKTFDEL 

       190        200        210        220        230        240 
IYLINHYSLI HRNIRFYFSL VSLQKNGAIE RKQMQETLDP KISRARSLSL LARLKKPVPL 

       250        260        270        280        290        300 
NFIVEENFVI DEKINLDLIL PRMVPESDVI NIKRRFKFLS VNERALSLNL ETGKTISKLL 

       310        320        330        340        350        360 
SSIYRDFSLL DPMVWFINLN CDTKLLDVNI EPEKNDVMIK SFEVIFKKIE NKLKLLLEER 

       370        380        390        400        410        420 
IGIETNMLGD KHVQPSINEK TSPALVIPTP DAENEISKGC GAVSGKDKTD IPQKNSDLIV 

       430        440        450        460        470        480 
PTFYDEANLE NTTIVAATPS PTKFSEDKAL DEQTQLTISS YRSSSSGSMA SEDSTNWRHN 

       490        500        510        520        530        540 
FQQELSENSE VAGAGSSTLP SSLTYNYIET IPENEDLELS KDASISNPFM ITKIRNVNKK 

       550        560        570        580        590        600 
LSENLLEAKR TSCGDANKRG MPNERQLSNT EKKELIKLQQ TYGKRNNTVD MTIPRNSKKK 

       610        620        630        640        650        660 
VTDNYIKKAS CMHKTRPKLM HFSEYTNNYV YTLKNEKIVK HDSDNFAKET LWLRSRDDAT 

       670        680        690 
SPSSSLLQAL RAHVKKPGHI EATTNEWCLF TPDSP 

References and documentation are available.

Number of amino acids: 695

Molecular weight: 78248.31

Theoretical pI: 8.31

Amino acid composition: 
Ala (A)  29	  4.2%
Arg (R)  33	  4.7%
Asn (N)  45	  6.5%
Asp (D)  41	  5.9%
Cys (C)   9	  1.3%
Gln (Q)  17	  2.4%
Glu (E)  49	  7.1%
Gly (G)  31	  4.5%
His (H)  10	  1.4%
Ile (I)  52	  7.5%
Leu (L)  71	 10.2%
Lys (K)  61	  8.8%
Met (M)  15	  2.2%
Phe (F)  22	  3.2%
Pro (P)  30	  4.3%
Ser (S)  73	 10.5%
Thr (T)  47	  6.8%
Trp (W)   6	  0.9%
Tyr (Y)  18	  2.6%
Val (V)  36	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 90
Total number of positively charged residues (Arg + Lys): 94

Atomic composition:

Carbon      C	      3450
Hydrogen    H	      5573
Nitrogen    N	       943
Oxygen      O	      1076
Sulfur      S	        24

Formula: C3450H5573N943O1076S24
Total number of atoms: 11066

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    60320
Abs 0.1% (=1 g/l)   0.771, assuming all pairs of Cys residues form cystines


Ext. coefficient    59820
Abs 0.1% (=1 g/l)   0.764, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.07
This classifies the protein as unstable.



Aliphatic index: 88.22

Grand average of hydropathicity (GRAVY): -0.448