ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTIPTDNNSS NNKGYNDNNN NNNNNNNNNN NNNNNNNNNN KEHPNINNNN NNNNNNNNNN 

        70         80         90        100        110        120 
IKESSSSNSN HSSSQSSSTA TVNSNPKVYP YEIIKQVGQG TFGKVYEAKN QDNKRVAIKK 

       130        140        150        160        170        180 
VEKSNHFISR EYDILKIVAH PNCLRILDMF YTAEDNKKMQ NLVFDFIPYT LASLLKKRQL 

       190        200        210        220        230        240 
SINFIKVLFY QLCQAIKHIH SKAICHRDIT PNNILLSSKG ELTLADFGSA KILESNHTSM 

       250        260        270        280        290        300 
SYICSRYYRA PELLVGCSNY TTKIDIWSIG CILAEMLIGK PLFPGTNSND QLGRIIEVLG 

       310        320        330        340        350        360 
SPTKDDMEAM KPSKPYHLQL PNINPKFFES LHNVEDKTVV DLLSKIFIFD PVKRASIDEI 

       370        380        390        400        410        420 
IAHPFLRDVN INSLELFDEM KCFSVSGNGK SSLTTNSTSS SSTTANMTSL ASSSSNNKTT 

       430        440        450        460        470 
CSETYLSRLP TSAITSSSNL KSIDNSNNGK SSSSSNNIPS LNNSNNGVIT NTI 

References and documentation are available.

Number of amino acids: 473

Molecular weight: 52553.51

Theoretical pI: 8.80

Amino acid composition: 
Ala (A)  18	  3.8%
Arg (R)  11	  2.3%
Asn (N)  81	 17.1%
Asp (D)  20	  4.2%
Cys (C)   8	  1.7%
Gln (Q)  10	  2.1%
Glu (E)  19	  4.0%
Gly (G)  16	  3.4%
His (H)  11	  2.3%
Ile (I)  39	  8.2%
Leu (L)  36	  7.6%
Lys (K)  35	  7.4%
Met (M)   9	  1.9%
Phe (F)  16	  3.4%
Pro (P)  19	  4.0%
Ser (S)  64	 13.5%
Thr (T)  28	  5.9%
Trp (W)   1	  0.2%
Tyr (Y)  14	  3.0%
Val (V)  18	  3.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 46

Atomic composition:

Carbon      C	      2266
Hydrogen    H	      3605
Nitrogen    N	       655
Oxygen      O	       749
Sulfur      S	        17

Formula: C2266H3605N655O749S17
Total number of atoms: 7292

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    26860
Abs 0.1% (=1 g/l)   0.511, assuming all pairs of Cys residues form cystines


Ext. coefficient    26360
Abs 0.1% (=1 g/l)   0.502, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.14
This classifies the protein as unstable.



Aliphatic index: 76.68

Grand average of hydropathicity (GRAVY): -0.636