ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTIRYWNIDL EEMLEAGVHF GHGTRKWNPK MAPYISAKRK GIHITNLTRT ARFLSEACNL 

        70         80         90        100        110        120 
VFDAASRGKQ FLIVGTKNKA ADSVAWAAIK ARCHFVNKKW LGGMLTNWST TEMRLHKFRD 

       130        140        150        160        170        180 
LRMEQKMGRF NRLPKRDAAV VKRQLSRLQT YLGGIKYMTG LPDIVIIVDQ HEEYTALREC 

       190        200        210        220        230        240 
ITLGIPTICL IDTNCDPDLA DISIPANDDA ISSIRLILNK LVFAICEGRS SYSSSYIRNP 


GLIIR 

References and documentation are available.

Number of amino acids: 245

Molecular weight: 27831.46

Theoretical pI: 9.64

Amino acid composition: 
Ala (A)  20	  8.2%
Arg (R)  20	  8.2%
Asn (N)  12	  4.9%
Asp (D)  13	  5.3%
Cys (C)   6	  2.4%
Gln (Q)   5	  2.0%
Glu (E)  10	  4.1%
Gly (G)  15	  6.1%
His (H)   6	  2.4%
Ile (I)  24	  9.8%
Leu (L)  23	  9.4%
Lys (K)  16	  6.5%
Met (M)   8	  3.3%
Phe (F)   8	  3.3%
Pro (P)   8	  3.3%
Ser (S)  14	  5.7%
Thr (T)  15	  6.1%
Trp (W)   5	  2.0%
Tyr (Y)   7	  2.9%
Val (V)  10	  4.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 23
Total number of positively charged residues (Arg + Lys): 36

Atomic composition:

Carbon      C	      1239
Hydrogen    H	      1993
Nitrogen    N	       355
Oxygen      O	       345
Sulfur      S	        14

Formula: C1239H1993N355O345S14
Total number of atoms: 3946

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    38305
Abs 0.1% (=1 g/l)   1.376, assuming all pairs of Cys residues form cystines


Ext. coefficient    37930
Abs 0.1% (=1 g/l)   1.363, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.92
This classifies the protein as unstable.



Aliphatic index: 94.82

Grand average of hydropathicity (GRAVY): -0.162