ProtParam
User-provided sequence:
10 20 30 40 50 60
MTISDHPETE PKWWKEATIY QIYPASFKDS NNDGWGDLKG ITSKLQYIKD LGVDAIWVCP
70 80 90 100 110 120
FYDSPQQDMG YDISNYEKVW PTYGTNEDCF ELIDKTHKLG MKFITDLVIN HCSTEHEWFK
130 140 150 160 170 180
ESRSSKTNPK RDWFFWRPPK GYDAEGKPIP PNNWKSFFGG SAWTFDETTN EFYLRLFASR
190 200 210 220 230 240
QVDLNWENED CRRAIFESAV GFWLDHGVDG FRIDTAGLYS KRPGLPDSPI FDKTSKLQHP
250 260 270 280 290 300
NWGSHNGPRI HEYHQELHRF MKNRVKDGRE IMTVGEVAHG SDNALYTSAA RYEVSEVFSF
310 320 330 340 350 360
THVELGTSPF FRYNIVPFTL KQWKEAIASN FLFINGTDSW ATTYIENHDQ ARSITRFADD
370 380 390 400 410 420
SPKYRKISGK LLTLLECSLT GTLYVYQGQE IGQINFKEWP IEKYEDVDVK NNYEIIKKSF
430 440 450 460 470 480
GKNSKEMKDF FKGIALLSRD HSRTPMPWTK DKPNAGFTGP DVKPWFFLNE SFEQGINVEQ
490 500 510 520 530 540
ESRDDDSVLN FWKRALQARK KYKELMIYGY DFQFIDLDSD QIFSFTKEYE DKTLFAALNF
550 560 570 580
SGEEIEFSLP REGASLSFIL GNYDDTDVSS RVLKPWEGRI YLVK
References and
documentation are available.
Number of amino acids: 584
Molecular weight: 68142.30
Theoretical pI: 5.47
Total number of negatively charged residues (Asp + Glu): 88
Total number of positively charged residues (Arg + Lys): 72
Atomic composition:
Carbon C 3105
Hydrogen H 4602
Nitrogen N 802
Oxygen O 910
Sulfur S 13
Formula: C
3105H
4602N
802O
910S
13
Total number of atoms: 9432
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 148990
Abs 0.1% (=1 g/l) 2.186, assuming all pairs of Cys residues form cystines
Ext. coefficient 148740
Abs 0.1% (=1 g/l) 2.183, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 30.29
This classifies the protein as stable.
Aliphatic index: 64.45
Grand average of hydropathicity (GRAVY): -0.670