ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTISNKSWRS YFPHLRKLPE DDQYLYSDDT NSSIIAEEEL HHSVDKSSKT DVTAETTAVE 

        70         80         90        100        110        120 
PHPHNLRHDL PYEVRDEAGR KWWKYFDEFE YRVNKEYKKS RKWYEFLYPN HTTQTKAERR 

       130        140        150        160        170        180 
LLYKLDIIIA LYFFMLCWSK SVDLNNYTNA YVSNMKEDLN MKGNDYVYTS TIANVGAIVF 

       190        200        210        220        230        240 
QLPFMYLLPR FPSHIILPVM DLGWTWFTFA CYRANSLAEL RAYRFILSAF GAAYYPVSQY 

       250        260        270        280        290        300 
ILGCWYAPDE INSRVCLFFC GQQLGSVTSG LLQSRIFKSL NGVHGLAGWR WMFLIDAIAI 

       310        320        330        340        350        360 
SLPTAIIGFF VIPGVPSKCY SLFLTDEEIR IARARNKRNQ IKDGVDKSKL APLWSRKLWK 

       370        380        390        400        410        420 
KVFCTPAFWV LVVFDTCSWN NMTAYSGSYT LWLKSNTKYS IAQVNNLSVI PACLGFAYVI 

       430        440        450        460        470        480 
FCAFGADLFR CKWIFMVFAA IMNTVSCALL IKWDIPSKAK WYAFFTTYFS VAASPCLWSF 

       490        500        510        520        530        540 
INDFLRFDPQ VKAITWIAIY SFSQSTYAWI PTLAWPTVES PRFKTGYTVS LIFGAIYGLW 

       550        560        570        580        590 
TFVVLFFYKR NEKKHALGNG IILYDSNKGE ELPEFVKKNM EERDGYYYLK RSS 

References and documentation are available.

Number of amino acids: 593

Molecular weight: 69082.80

Theoretical pI: 9.02

Amino acid composition: 
Ala (A)  44	  7.4%
Arg (R)  27	  4.6%
Asn (N)  28	  4.7%
Asp (D)  26	  4.4%
Cys (C)  13	  2.2%
Gln (Q)  11	  1.9%
Glu (E)  25	  4.2%
Gly (G)  25	  4.2%
His (H)  10	  1.7%
Ile (I)  39	  6.6%
Leu (L)  54	  9.1%
Lys (K)  39	  6.6%
Met (M)  11	  1.9%
Phe (F)  42	  7.1%
Pro (P)  25	  4.2%
Ser (S)  47	  7.9%
Thr (T)  32	  5.4%
Trp (W)  23	  3.9%
Tyr (Y)  38	  6.4%
Val (V)  34	  5.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 66

Atomic composition:

Carbon      C	      3223
Hydrogen    H	      4797
Nitrogen    N	       795
Oxygen      O	       852
Sulfur      S	        24

Formula: C3223H4797N795O852S24
Total number of atoms: 9691

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   183870
Abs 0.1% (=1 g/l)   2.662, assuming all pairs of Cys residues form cystines


Ext. coefficient   183120
Abs 0.1% (=1 g/l)   2.651, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.27
This classifies the protein as unstable.



Aliphatic index: 85.21

Grand average of hydropathicity (GRAVY): -0.046