ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTISTLSNET TKSGSCSGQG KNGGKDFTYG KKCFTKEEWK EQVAKYSAMG ELYANKTIHY 

        70         80         90        100        110        120 
PLKIQPNSSG GSQDEGFATI QTTPIEPTLP RLLLNYFVSM AYEDSSIRMA KELGFIRNNK 

       130        140        150        160        170        180 
DIAVFNDLYK IKERFHIKHL IKLGRINEAM EEINSIFGLE VLEETFNATG SYTGRTDRQQ 

       190        200        210        220        230        240 
QQQQQQFDID GDLHFKLLLL NLIEMIRSHH QQENITKDSN DFILNLIQYS QNKLAIKASS 

       250        260        270        280        290        300 
SVKKMQELEL AMTLLLFPLS DSADSGSIKL PKSLQNLYSI SLRSKIADLV NEKLLKFIHP 

       310        320        330        340        350        360 
RIQFEISNNN SKFPDLLNSD KKIITQNFTV YNNNLVNGSN GTKITHISSD QPINEKMSSN 

       370        380        390        400        410        420 
EVTAAANSVW LNQRDGNVGT GSAATTFHNL ENKNYWNQTS ELLSSSNGKE KGLEFNNYYS 

       430        440        450 
SEFPYEPRLT QIMKLWCWCE NQLHHNQIGV PRVEN 

References and documentation are available.

Number of amino acids: 455

Molecular weight: 51729.34

Theoretical pI: 6.85

Amino acid composition: 
Ala (A)  19	  4.2%
Arg (R)  13	  2.9%
Asn (N)  43	  9.5%
Asp (D)  18	  4.0%
Cys (C)   4	  0.9%
Gln (Q)  27	  5.9%
Glu (E)  32	  7.0%
Gly (G)  25	  5.5%
His (H)  11	  2.4%
Ile (I)  34	  7.5%
Leu (L)  46	 10.1%
Lys (K)  36	  7.9%
Met (M)  10	  2.2%
Phe (F)  20	  4.4%
Pro (P)  13	  2.9%
Ser (S)  44	  9.7%
Thr (T)  27	  5.9%
Trp (W)   5	  1.1%
Tyr (Y)  15	  3.3%
Val (V)  13	  2.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 50
Total number of positively charged residues (Arg + Lys): 49

Atomic composition:

Carbon      C	      2288
Hydrogen    H	      3597
Nitrogen    N	       627
Oxygen      O	       712
Sulfur      S	        14

Formula: C2288H3597N627O712S14
Total number of atoms: 7238

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    50100
Abs 0.1% (=1 g/l)   0.969, assuming all pairs of Cys residues form cystines


Ext. coefficient    49850
Abs 0.1% (=1 g/l)   0.964, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.66
This classifies the protein as stable.



Aliphatic index: 81.03

Grand average of hydropathicity (GRAVY): -0.575