ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTITAPPKDE IISKFPGSEP LLNFYNELSD AEKSKLFHQI STLNLSEAHQ WFIDSADQRA 

        70         80         90        100        110        120 
PSTAEDLKPV LDSQHFVQAE LHQVILDGLW NKGMDAIGRG EVCAIVLAGG QATRLGSSQP 

       130        140        150        160        170        180 
KGTIPLGINA SFGDSLLGIQ AAKIALLQAL AGEREHQNPG KIHWAVMTSP GTEEATREHV 

       190        200        210        220        230        240 
KKLAAHHGFD FDEQITIFSQ DEIAAYDEQG NFLLGTKGSV VAAPNGNGGL YSAISAHLPR 

       250        260        270        280        290        300 
LRAKGIKYFH VYCVDNILCK VADPHFIGFA ISNEADVATK CVPKQKGELV GSVCLDRGLP 

       310        320        330        340        350        360 
RVVEYSELGA ELAEQKTPDG KYLFGAGSIA NHFFTMDFMD RVCSPSSRLP YHRAHKKISY 

       370        380        390        400        410        420 
VNEQGTIVKP EKPNGIKLEQ FIFDVFELSK RFFIWEVARN EEFSPLKNAQ SVGTDCLSTC 

       430        440        450        460        470        480 
QRDLSNVNKL WLERVQAKVT ATEKPIYLKT IVSYNGENLQ ELRHREISDS ALESDHSINK 


FFVV 

References and documentation are available.

Number of amino acids: 484

Molecular weight: 53496.71

Theoretical pI: 5.96

Amino acid composition: 
Ala (A)  42	  8.7%
Arg (R)  18	  3.7%
Asn (N)  21	  4.3%
Asp (D)  25	  5.2%
Cys (C)   8	  1.7%
Gln (Q)  22	  4.5%
Glu (E)  35	  7.2%
Gly (G)  35	  7.2%
His (H)  17	  3.5%
Ile (I)  31	  6.4%
Leu (L)  44	  9.1%
Lys (K)  32	  6.6%
Met (M)   5	  1.0%
Phe (F)  25	  5.2%
Pro (P)  22	  4.5%
Ser (S)  36	  7.4%
Thr (T)  20	  4.1%
Trp (W)   5	  1.0%
Tyr (Y)  11	  2.3%
Val (V)  30	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 60
Total number of positively charged residues (Arg + Lys): 50

Atomic composition:

Carbon      C	      2393
Hydrogen    H	      3736
Nitrogen    N	       652
Oxygen      O	       715
Sulfur      S	        13

Formula: C2393H3736N652O715S13
Total number of atoms: 7509

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    44390
Abs 0.1% (=1 g/l)   0.830, assuming all pairs of Cys residues form cystines


Ext. coefficient    43890
Abs 0.1% (=1 g/l)   0.820, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 28.55
This classifies the protein as stable.



Aliphatic index: 87.09

Grand average of hydropathicity (GRAVY): -0.256