ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTKAVDGLTF PMSRVSESAS RLKTIISDML PRFTDDYSDD LSEYVTVLVC NGKSQRQASE 

        70         80         90        100        110        120 
DLEAFLKEQS WKFVVCLWEL LVKYFSQSNS ASGPKTDVDF GSNDTLIKQG LSSRKYDDYD 

       130        140        150        160        170        180 
CKAADGTNSM NEQLISVTAP IDDIEAPVSP KVEKMKMSRQ ELIDSPCTRA RRRKKENWNS 

       190        200        210        220        230        240 
SGYSRKVLRS VIVSATQQPC DRNPAKYEKS MNERSRTLKK RPYSPERELD SRFAPSGRAV 

       250        260        270        280        290        300 
SARYHDASLH QETEPHVSVW DRLGRASSEC VLDTESRTLS KFGIQTRETK VHQQHGPVFP 

       310        320        330        340        350        360 
AAYSEQHSEI FQREVLAVGY RHRVPQFDKD RKPKSETITS TEPHIAYNLS RKRRYGMVSP 

       370        380        390        400        410        420 
NSGEFSYVPQ YKQAEQDFEK PSLLSHQSTK QDIFSEIKNV KEKMQELELR IIQSKQLKKQ 

       430        440        450        460        470        480 
KVEERKPSPQ SGESQYQEDV SESRVIHVTN VYYAAKKEAI SMFFSSKCGA VQNVIVVTDP 

       490        500        510        520        530 
VTRHPKGTAF VTFATKESVG KAVALSGTMF YSRPIKVRMH VIASGVVSAA PQIVTGS 

References and documentation are available.

Number of amino acids: 537

Molecular weight: 60796.65

Theoretical pI: 9.20

Amino acid composition: 
Ala (A)  33	  6.1%
Arg (R)  37	  6.9%
Asn (N)  14	  2.6%
Asp (D)  28	  5.2%
Cys (C)   7	  1.3%
Gln (Q)  30	  5.6%
Glu (E)  39	  7.3%
Gly (G)  21	  3.9%
His (H)  12	  2.2%
Ile (I)  22	  4.1%
Leu (L)  29	  5.4%
Lys (K)  44	  8.2%
Met (M)  12	  2.2%
Phe (F)  19	  3.5%
Pro (P)  27	  5.0%
Ser (S)  66	 12.3%
Thr (T)  29	  5.4%
Trp (W)   4	  0.7%
Tyr (Y)  19	  3.5%
Val (V)  45	  8.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 67
Total number of positively charged residues (Arg + Lys): 81

Atomic composition:

Carbon      C	      2659
Hydrogen    H	      4236
Nitrogen    N	       764
Oxygen      O	       830
Sulfur      S	        19

Formula: C2659H4236N764O830S19
Total number of atoms: 8508

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    50685
Abs 0.1% (=1 g/l)   0.834, assuming all pairs of Cys residues form cystines


Ext. coefficient    50310
Abs 0.1% (=1 g/l)   0.828, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 58.04
This classifies the protein as unstable.



Aliphatic index: 67.49

Grand average of hydropathicity (GRAVY): -0.683