ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTKAYTIVIK LGSSSLVDES TKEPKLSIMT LIVETVTNLK RMGHKVIIVS SGGIAVGLDA 

        70         80         90        100        110        120 
LNIPHKPKQL SEVQAIAAVG QGRLIARWNM LFSQYGEQTA QILLTRNDIL RWNQYNNARN 

       130        140        150        160        170        180 
TINELLAMGV IPIVNENDTL SISEIEFGDN DTLSAITAAL VGADFLFLLT DVDCLYTDNP 

       190        200        210        220        230        240 
RTNPDARPIV LVPELSEGLP GVNTSSGSGS EVGTGGMRTK LIAADLASNA GIETIVMKSD 

       250        260        270        280        290        300 
RPEYVPKIVD YIQHHFRPPR HIGNGTQQQF LDLQDTELEQ LRRYDVPMHT KFLANDNKHK 

       310        320        330        340        350        360 
LKNREFWILH GLITKGAIII DENSYDKLLS KDMASLTPNA VIEVRDNFHE LECVDLKIGQ 

       370        380        390        400        410        420 
RLPNGELDIS KPIQSVGCVR SNYTSLELAK IKGLPSEKIH DVLGYSVSEY VAHRENIAFP 


PQF 

References and documentation are available.

Number of amino acids: 423

Molecular weight: 46863.62

Theoretical pI: 5.71

Amino acid composition: 
Ala (A)  26	  6.1%
Arg (R)  19	  4.5%
Asn (N)  26	  6.1%
Asp (D)  25	  5.9%
Cys (C)   3	  0.7%
Gln (Q)  16	  3.8%
Glu (E)  26	  6.1%
Gly (G)  28	  6.6%
His (H)  11	  2.6%
Ile (I)  37	  8.7%
Leu (L)  46	 10.9%
Lys (K)  23	  5.4%
Met (M)   9	  2.1%
Phe (F)  11	  2.6%
Pro (P)  20	  4.7%
Ser (S)  29	  6.9%
Thr (T)  25	  5.9%
Trp (W)   3	  0.7%
Tyr (Y)  11	  2.6%
Val (V)  29	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 42

Atomic composition:

Carbon      C	      2081
Hydrogen    H	      3346
Nitrogen    N	       570
Oxygen      O	       633
Sulfur      S	        12

Formula: C2081H3346N570O633S12
Total number of atoms: 6642

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    33015
Abs 0.1% (=1 g/l)   0.704, assuming all pairs of Cys residues form cystines


Ext. coefficient    32890
Abs 0.1% (=1 g/l)   0.702, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.28
This classifies the protein as stable.



Aliphatic index: 102.55

Grand average of hydropathicity (GRAVY): -0.169