ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTKETIRVVI CGDEGVGKSS LIVSLTKAEF IPTIQDVLPP ISIPRDFSSS PTYSPKNTVL 

        70         80         90        100        110        120 
IDTSDSDLIA LDHELKSADV IWLVYCDHES YDHVSLFWLP HFRSLGLNIP VILCKNKCDS 

       130        140        150        160        170        180 
ISNVNANAMV VSENSDDDID TKVEDEEFIP ILMEFKEIDT CIKTSAKTQF DLNQAFYLCQ 

       190        200        210        220        230        240 
RAITHPISPL FDAMVGELKP LAVMALKRIF LLSDLNQDSY LDDNEILGLQ KKCFNKSIDV 

       250        260        270        280        290        300 
NELNFIKDLL LDISKHDQEY INRKLYVPGK GITKDGFLVL NKIYAERGRH ETTWAILRTF 

       310        320        330        340        350        360 
HYTDSLCIND KILHPRLVVP DTSSVELSPK GYRFLVDIFL KFDIDNDGGL NNQELHRLFK 

       370        380        390        400        410        420 
CTPGLPKLWT STNFPFSTVV NNKGCITLQG WLAQWSMTTF LNYSTTTAYL VYFGFQEDAR 

       430        440        450        460        470        480 
LALQVTKPRK MRRRSGKLYR SNINDRKVFN CFVIGKPCCG KSSLLEAFLG RSFSEEYSPT 

       490        500        510        520        530        540 
IKPRIAVNSL ELKGGKQYYL ILQELGEQEY AILENKDKLK ECDVICLTYD SSDPESFSYL 

       550        560        570        580        590        600 
VSLLDKFTHL QDLPLVFVAS KADLDKQQQR CQIQPDELAD ELFVNHPLHI SSRWLSSLNE 

       610        620        630        640        650        660 
LFIKITEAAL DPGKNTPGLP EETAAKDVDY RQTALIFGST VGFVALCSFT LMKLFKSSKF 


SK 

References and documentation are available.

Number of amino acids: 662

Molecular weight: 75150.33

Theoretical pI: 5.66

Amino acid composition: 
Ala (A)  29	  4.4%
Arg (R)  24	  3.6%
Asn (N)  31	  4.7%
Asp (D)  50	  7.6%
Cys (C)  17	  2.6%
Gln (Q)  22	  3.3%
Glu (E)  36	  5.4%
Gly (G)  28	  4.2%
His (H)  13	  2.0%
Ile (I)  46	  6.9%
Leu (L)  82	 12.4%
Lys (K)  50	  7.6%
Met (M)   8	  1.2%
Phe (F)  37	  5.6%
Pro (P)  30	  4.5%
Ser (S)  55	  8.3%
Thr (T)  38	  5.7%
Trp (W)   7	  1.1%
Tyr (Y)  21	  3.2%
Val (V)  38	  5.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 86
Total number of positively charged residues (Arg + Lys): 74

Atomic composition:

Carbon      C	      3394
Hydrogen    H	      5324
Nitrogen    N	       870
Oxygen      O	      1002
Sulfur      S	        25

Formula: C3394H5324N870O1002S25
Total number of atoms: 10615

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    70790
Abs 0.1% (=1 g/l)   0.942, assuming all pairs of Cys residues form cystines


Ext. coefficient    69790
Abs 0.1% (=1 g/l)   0.929, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.83
This classifies the protein as stable.



Aliphatic index: 96.44

Grand average of hydropathicity (GRAVY): -0.155