ProtParam
User-provided sequence:
10 20 30 40 50 60
MTKEVPYYCD NDDNNIIRLF IIRHGQTEHN VKKILQGHKD TSINPTGEEQ ATKLGHYLRS
70 80 90 100 110 120
RGIHFDKVVS SDLKRCRQTT ALVLKHSKQE NVPTSYTSGL RERYMGVIEG MQITEAEKYA
130 140 150 160 170 180
DKHGEGSFRN FGEKSDDFVA RLTGCVEEEV AEASNEGVKN LALVSHGGAI RMILQWLKYE
190 200 210 220 230
NHQAHKIIVF NTSVTIVDYV KDSKQFIVRR VGNTQHLGDG EFVVSDLRLR
References and
documentation are available.
Number of amino acids: 230
Molecular weight: 26176.58
Theoretical pI: 7.78
Total number of negatively charged residues (Asp + Glu): 31
Total number of positively charged residues (Arg + Lys): 32
Atomic composition:
Carbon C 1147
Hydrogen H 1825
Nitrogen N 337
Oxygen O 351
Sulfur S 7
Formula: C
1147H
1825N
337O
351S
7
Total number of atoms: 3667
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 17545
Abs 0.1% (=1 g/l) 0.670, assuming all pairs of Cys residues form cystines
Ext. coefficient 17420
Abs 0.1% (=1 g/l) 0.665, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 37.42
This classifies the protein as stable.
Aliphatic index: 82.13
Grand average of hydropathicity (GRAVY): -0.570