ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTKIKVANPI VEMDGDEQTR IIWHLIRDKL VLPYLDVDLK YYDLSVEYRD QTNDQVTVDS 

        70         80         90        100        110        120 
ATATLKYGVA VKCATITPDE ARVEEFHLKK MWKSPNGTIR NILGGTVFRE PIIIPRIPRL 

       130        140        150        160        170        180 
VPQWEKPIII GRHAFGDQYK ATDVIVPEEG ELRLVYKSKS GTHDVDLKVF DYPEHGGVAM 

       190        200        210        220        230        240 
MMYNTTDSIE GFAKASFELA IERKLPLYST TKNTILKKYD GKFKDVFEAM YARSYKEKFE 

       250        260        270        280        290        300 
SLGIWYEHRL IDDMVAQMLK SKGGYIIAMK NYDGDVESDI VAQGFGSLGL MTSVLITPDG 

       310        320        330        340        350        360 
KTFESEAAHG TVTRHFRQHQ QGKETSTNSI ASIFAWTRGI IQRGKLDNTP DVVKFGQILE 

       370        380        390        400        410 
SATVNTVQED GIMTKDLALI LGKSERSAYV TTEEFIDAVE SRLKKEFEAA AL 

References and documentation are available.

Number of amino acids: 412

Molecular weight: 46562.28

Theoretical pI: 5.82

Amino acid composition: 
Ala (A)  28	  6.8%
Arg (R)  19	  4.6%
Asn (N)  10	  2.4%
Asp (D)  29	  7.0%
Cys (C)   1	  0.2%
Gln (Q)  13	  3.2%
Glu (E)  31	  7.5%
Gly (G)  28	  6.8%
His (H)   9	  2.2%
Ile (I)  33	  8.0%
Leu (L)  30	  7.3%
Lys (K)  34	  8.3%
Met (M)  12	  2.9%
Phe (F)  16	  3.9%
Pro (P)  14	  3.4%
Ser (S)  22	  5.3%
Thr (T)  31	  7.5%
Trp (W)   5	  1.2%
Tyr (Y)  17	  4.1%
Val (V)  30	  7.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 60
Total number of positively charged residues (Arg + Lys): 53

Atomic composition:

Carbon      C	      2091
Hydrogen    H	      3299
Nitrogen    N	       549
Oxygen      O	       626
Sulfur      S	        13

Formula: C2091H3299N549O626S13
Total number of atoms: 6578

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    52830
Abs 0.1% (=1 g/l)   1.135, assuming all pairs of Cys residues form cystines


Ext. coefficient    52830
Abs 0.1% (=1 g/l)   1.135, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 24.34
This classifies the protein as stable.



Aliphatic index: 87.55

Grand average of hydropathicity (GRAVY): -0.310