ProtParam
User-provided sequence:
10 20 30 40 50 60
MTKKDKKEVK VQTVTTEDGE TVKVFEDLQG FETFIANETE DDDFDHLHCK LNYYPPFVLH
70 80 90 100 110 120
ESHEDPEKIS DAANSHSKKF VRHLHQHIEK HLLKDIKQAV RKPELKFHEK SKEETFDKIT
130 140 150 160
WHYGEETEYH GRPFKIDVQV VCTHEDAMVF VDYKTHPVGA N
References and
documentation are available.
Number of amino acids: 161
Molecular weight: 18989.23
Theoretical pI: 5.74
Total number of negatively charged residues (Asp + Glu): 33
Total number of positively charged residues (Arg + Lys): 23
Atomic composition:
Carbon C 851
Hydrogen H 1292
Nitrogen N 228
Oxygen O 259
Sulfur S 4
Formula: C
851H
1292N
228O
259S
4
Total number of atoms: 2634
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 13075
Abs 0.1% (=1 g/l) 0.689, assuming all pairs of Cys residues form cystines
Ext. coefficient 12950
Abs 0.1% (=1 g/l) 0.682, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 40.22
This classifies the protein as unstable.
Aliphatic index: 62.86
Grand average of hydropathicity (GRAVY): -0.937