ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTKLHFDTAE PVKITLPNGL TYEQPTGLFI NNKFMKAQDG KTYPVEDPST ENTVCEVSSA 

        70         80         90        100        110        120 
TTEDVEYAIE CADRAFHDTE WATQDPRERG RLLSKLADEL ESQIDLVSSI EALDNGKTLA 

       130        140        150        160        170        180 
LARGDVTIAI NCLRDAAAYA DKVNGRTINT GDGYMNFTTL EPIGVCGQII PWNFPIMMLA 

       190        200        210        220        230        240 
WKIAPALAMG NVCILKPAAV TPLNALYFAS LCKKVGIPAG VVNIVPGPGR TVGAALTNDP 

       250        260        270        280        290        300 
RIRKLAFTGS TEVGKSVAVD SSESNLKKIT LELGGKSAHL VFDDANIKKT LPNLVNGIFK 

       310        320        330        340        350        360 
NAGQICSSGS RIYVQEGIYD ELLAAFKAYL ETEIKVGNPF DKANFQGAIT NRQQFDTIMN 

       370        380        390        400        410        420 
YIDIGKKEGA KILTGGEKVG DKGYFIRPTV FYDVNEDMRI VKEEIFGPVV TVAKFKTLEE 

       430        440        450        460        470        480 
GVEMANSSEF GLGSGIETES LSTGLKVAKM LKAGTVWINT YNDFDSRVPF GGVKQSGYGR 

       490        500 
EMGEEVYHAY TEVKAVRIKL 

References and documentation are available.

Number of amino acids: 500

Molecular weight: 54414.16

Theoretical pI: 5.31

Amino acid composition: 
Ala (A)  44	  8.8%
Arg (R)  17	  3.4%
Asn (N)  27	  5.4%
Asp (D)  27	  5.4%
Cys (C)   7	  1.4%
Gln (Q)  11	  2.2%
Glu (E)  36	  7.2%
Gly (G)  45	  9.0%
His (H)   4	  0.8%
Ile (I)  35	  7.0%
Leu (L)  38	  7.6%
Lys (K)  37	  7.4%
Met (M)  11	  2.2%
Phe (F)  21	  4.2%
Pro (P)  21	  4.2%
Ser (S)  24	  4.8%
Thr (T)  37	  7.4%
Trp (W)   4	  0.8%
Tyr (Y)  16	  3.2%
Val (V)  38	  7.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 54

Atomic composition:

Carbon      C	      2427
Hydrogen    H	      3848
Nitrogen    N	       638
Oxygen      O	       742
Sulfur      S	        18

Formula: C2427H3848N638O742S18
Total number of atoms: 7673

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    46215
Abs 0.1% (=1 g/l)   0.849, assuming all pairs of Cys residues form cystines


Ext. coefficient    45840
Abs 0.1% (=1 g/l)   0.842, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 28.04
This classifies the protein as stable.



Aliphatic index: 87.78

Grand average of hydropathicity (GRAVY): -0.141