ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTKLLEINHI QTLCFAKGFS PARLNVATSP FRISRRGGGG YCSNACIPYR LKFTCYATLS 

        70         80         90        100        110        120 
AVVKEQASDF SGKEAALLVD ELRSNFNSGR TKSYEWRISQ LQNIARMIDE KEKCITEALY 

       130        140        150        160        170        180 
QDLSKPELEA FLAEISNTKS SCMLAIKELK NWMAPETVKT SVTTFPSSAQ IVSEPLGVVL 

       190        200        210        220        230        240 
VISAWNFPFL LSVEPVIGAI AAGNAVVLKP SEIAPAASSL LAKLFSEYLD NTTIRVIEGG 

       250        260        270        280        290        300 
VPETTALLDQ KWDKIFFTGG ARVARIIMAA AARNLTPVVL ELGGKCPALV DSDVNLQVAA 

       310        320        330        340        350        360 
RRIIAGKWAC NSGQACIGVD YVITTKDFAS KLIDALKTEL ETFFGQNALE SKDLSRIVNS 

       370        380        390        400        410        420 
FHFKRLESML KENGVANKIV HGGRITEDKL KISPTILLDV PEASSMMQEE IFGPLLPIIT 

       430        440        450        460        470        480 
VQKIEDGFQV IRSKPKPLAA YLFTNNKELE KQFVQDVSAG GITINDTVLH VTVKDLPFGG 

       490        500        510        520        530        540 
VGESGIGAYH GKFSYETFSH KKGVLYRSFS GDADLRYPPY TPKKKMVLKA LLSSNIFAAI 

       550 
LAFFGFSKDS 

References and documentation are available.

Number of amino acids: 550

Molecular weight: 60172.67

Theoretical pI: 8.74

Amino acid composition: 
Ala (A)  52	  9.5%
Arg (R)  21	  3.8%
Asn (N)  22	  4.0%
Asp (D)  22	  4.0%
Cys (C)   9	  1.6%
Gln (Q)  15	  2.7%
Glu (E)  34	  6.2%
Gly (G)  36	  6.5%
His (H)   6	  1.1%
Ile (I)  40	  7.3%
Leu (L)  55	 10.0%
Lys (K)  42	  7.6%
Met (M)   9	  1.6%
Phe (F)  30	  5.5%
Pro (P)  24	  4.4%
Ser (S)  46	  8.4%
Thr (T)  31	  5.6%
Trp (W)   5	  0.9%
Tyr (Y)  13	  2.4%
Val (V)  38	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 56
Total number of positively charged residues (Arg + Lys): 63

Atomic composition:

Carbon      C	      2719
Hydrogen    H	      4333
Nitrogen    N	       709
Oxygen      O	       790
Sulfur      S	        18

Formula: C2719H4333N709O790S18
Total number of atoms: 8569

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    47370
Abs 0.1% (=1 g/l)   0.787, assuming all pairs of Cys residues form cystines


Ext. coefficient    46870
Abs 0.1% (=1 g/l)   0.779, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.30
This classifies the protein as unstable.



Aliphatic index: 96.85

Grand average of hydropathicity (GRAVY): 0.055