ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTKQAEETQK PIIFAPETYQ YDKFTLNEKQ LTDDPIDLFT KWFNEAKEDP RETLPEAITF 

        70         80         90        100        110        120 
SSAELPSGRV SSRILLFKEL DHRGFTIYSN WGTSRKAHDI ATNPNAAIVF FWKDLQRQVR 

       130        140        150        160        170        180 
VEGITEHVNR ETSERYFKTR PRGSKIGAWA SRQSDVIKNR EELDELTQKN TERFKDAEDI 

       190        200        210        220 
PCPDYWGGLR IVPLEIEFWQ GRPSRLHDRF VYRRKTENDP WKVVRLAP 

References and documentation are available.

Number of amino acids: 228

Molecular weight: 26908.26

Theoretical pI: 6.94

Amino acid composition: 
Ala (A)  13	  5.7%
Arg (R)  21	  9.2%
Asn (N)   9	  3.9%
Asp (D)  15	  6.6%
Cys (C)   1	  0.4%
Gln (Q)   9	  3.9%
Glu (E)  22	  9.6%
Gly (G)   9	  3.9%
His (H)   4	  1.8%
Ile (I)  14	  6.1%
Leu (L)  15	  6.6%
Lys (K)  16	  7.0%
Met (M)   1	  0.4%
Phe (F)  13	  5.7%
Pro (P)  14	  6.1%
Ser (S)  12	  5.3%
Thr (T)  17	  7.5%
Trp (W)   7	  3.1%
Tyr (Y)   6	  2.6%
Val (V)  10	  4.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 37

Atomic composition:

Carbon      C	      1208
Hydrogen    H	      1862
Nitrogen    N	       340
Oxygen      O	       356
Sulfur      S	         2

Formula: C1208H1862N340O356S2
Total number of atoms: 3768

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    47440
Abs 0.1% (=1 g/l)   1.763, assuming all pairs of Cys residues form cystines


Ext. coefficient    47440
Abs 0.1% (=1 g/l)   1.763, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.64
This classifies the protein as stable.



Aliphatic index: 68.03

Grand average of hydropathicity (GRAVY): -0.867