ProtParam
User-provided sequence:
10 20 30 40 50 60
MTKQQTSVMR NASIAKEERE GSDNNNVDRS SSDAISDNDA ERSNSHSEID NESNFDMVPY
70 80 90 100 110 120
SRFSHKQKML LVVQCAFTGF FSTVAGSIYY PVLTIIERKF NITEELANVT IVVYFIFQGV
130 140 150 160 170 180
APSIMGGLAD TFGRRPIVLW AILAYFCACI GLACAHNYAQ ILALRCLQAA GISPVIAINS
190 200 210 220 230 240
GIMGDVTTKV ERGGYVGLVA GFQVVGTAFG ALIGAGLSSK WGWRAIFWFL AIGSGICLVF
250 260 270 280 290 300
STLLMPETKR TLVGNGSVTP RSFLNRSLIL HVGSVKKTLH LDDPDPETLE PRTSVDFLAP
310 320 330 340 350 360
LKILHIREID ILLSIAGLQF STWTTHQTAL TIVLSKKYNL SVAKIGLCFL PAGISTLTSI
370 380 390 400 410 420
ISAGRYLNWS YRTRKVKYNR WIKEQELQLM EKYKGDKNKV AELIHSNSHY AFNLVEARLH
430 440 450 460 470 480
PAFVTLLLSS IGFTAFGWCI SVKTPLAAVL CTSAFASLFS NCILTFSTTL IVDLFPSKAS
490 500 510 520 530 540
TATGCLNLFR CLLSAIFIAA LTKMVEKMRY GGVFTFLSAI TSSSSLLLFY LLKNGKQLSF
550 560
DRIRANDKSA GRSVGKNSEK VST
References and
documentation are available.
Number of amino acids: 563
Molecular weight: 61758.76
Theoretical pI: 9.32
Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 56
Atomic composition:
Carbon C 2798
Hydrogen H 4433
Nitrogen N 737
Oxygen O 791
Sulfur S 22
Formula: C
2798H
4433N
737O
791S
22
Total number of atoms: 8781
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 67100
Abs 0.1% (=1 g/l) 1.086, assuming all pairs of Cys residues form cystines
Ext. coefficient 66350
Abs 0.1% (=1 g/l) 1.074, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.18
This classifies the protein as stable.
Aliphatic index: 102.22
Grand average of hydropathicity (GRAVY): 0.266