ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTKSRKQKQK KQDFLRKKLK VGKPKEKARN ATDTSFVSKT ISIRNQHLDQ NPHDLTKRLT 

        70         80         90        100        110        120 
LLKHHNINVR KETLTTFQKS IPSIIKSRLM TPLLTQSIPL ICDESQQVRQ GLIDLVDEIG 

       130        140        150        160        170        180 
SHDAEILKLH CNIFVLYINM AMTHIVTQIQ ADSTKFLSHL LKYCGDEVVR KSWVKLLNGV 

       190        200        210        220        230        240 
FGVLGWGQVG KNDSASIVQT KKRNAKYVTI HLNALYTLVE YGCQDERARS DGDTAETTED 

       250        260        270        280        290        300 
SGTLRNPYLI PDYPQPFEHL KLFTRELKVQ DATSSGVNAT LLSLATQDID TRKAVFIEQF 

       310        320        330 
LPIVRKKIEV IIKEGGECGK SANKLKTLLA KIFD 

References and documentation are available.

Number of amino acids: 334

Molecular weight: 37866.88

Theoretical pI: 9.54

Amino acid composition: 
Ala (A)  16	  4.8%
Arg (R)  16	  4.8%
Asn (N)  14	  4.2%
Asp (D)  20	  6.0%
Cys (C)   5	  1.5%
Gln (Q)  19	  5.7%
Glu (E)  16	  4.8%
Gly (G)  16	  4.8%
His (H)  10	  3.0%
Ile (I)  25	  7.5%
Leu (L)  38	 11.4%
Lys (K)  36	 10.8%
Met (M)   4	  1.2%
Phe (F)  11	  3.3%
Pro (P)  10	  3.0%
Ser (S)  21	  6.3%
Thr (T)  27	  8.1%
Trp (W)   2	  0.6%
Tyr (Y)   7	  2.1%
Val (V)  21	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 52

Atomic composition:

Carbon      C	      1686
Hydrogen    H	      2761
Nitrogen    N	       473
Oxygen      O	       495
Sulfur      S	         9

Formula: C1686H2761N473O495S9
Total number of atoms: 5424

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    21680
Abs 0.1% (=1 g/l)   0.573, assuming all pairs of Cys residues form cystines


Ext. coefficient    21430
Abs 0.1% (=1 g/l)   0.566, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.54
This classifies the protein as stable.



Aliphatic index: 96.59

Grand average of hydropathicity (GRAVY): -0.390