ProtParam
User-provided sequence:
10 20 30 40 50 60
MTKSVDTLLI PGPIILSGAV QKALDVPSLG HTSPEFVSIF QRVLKNTRAV FKSAAASKSQ
70 80 90 100 110 120
PFVLAGSGTL GWDIFASNFI LSKAPNKNVL VVSTGTFSDR FADCLRSYGA QVDVVRPLKI
130 140 150 160 170 180
GESVPLELIT EKLSQNSYGA VTVTHVDTST AVLSDLKAIS QAIKQTSPET FFVVDAVCSI
190 200 210 220 230 240
GCEEFEFDEW GVDFALTASQ KAIGAPAGLS ISLCSSRFMD YALNDSKNGH VHGYFSSLRR
250 260 270 280 290 300
WTPIMENYEA GKGAYFATPP VQLINSLDVA LKEILEEGLH KRWDLHREMS DWFKDSLVNG
310 320 330 340 350 360
LQLTSVSRYP SNMSAHGLTA VYVADPPDVI AFLKSHGVVI AGGIHKDIGP KYIRIGHMGV
370 380
TACNKNLPYM KNCFDLIKLA LQRKK
References and
documentation are available.
Number of amino acids: 385
Molecular weight: 41907.18
Theoretical pI: 8.12
Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 38
Atomic composition:
Carbon C 1889
Hydrogen H 2973
Nitrogen N 499
Oxygen O 551
Sulfur S 13
Formula: C
1889H
2973N
499O
551S
13
Total number of atoms: 5925
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 42775
Abs 0.1% (=1 g/l) 1.021, assuming all pairs of Cys residues form cystines
Ext. coefficient 42400
Abs 0.1% (=1 g/l) 1.012, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 34.96
This classifies the protein as stable.
Aliphatic index: 94.21
Grand average of hydropathicity (GRAVY): 0.054