ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTKTITVAHI QYDFKAVLEE NDENDDEFYI NVDKNLNEIK EHKIVVLGNS RGVDAGKGNT 

        70         80         90        100        110        120 
FEKVGSHLYK ARLDGHDFLF NTIIRDGSKM LKRADYTAVD TAKLQMRRFI LGTTEGDIKV 

       130        140        150        160        170        180 
LDSNFNLQRE IDQAHVSEIT KLKFFPSGEA LISSSQDMQL KIWSVKDGSN PRTLIGHRAT 

       190        200        210        220        230        240 
VTDIAIIDRG RNVLSASLDG TIRLWECGTG TTIHTFNRKE NPHDGVNSIA LFVGTDRQLH 

       250        260        270        280        290        300 
EISTSKKNNL EFGTYGKYVI AGHVSGVITV HNVFSKEQTI QLPSKFTCSC NSLTVDGNNA 

       310        320        330        340        350        360 
NYIYAGYENG MLAQWDLRSP ECPVGEFLIN EGTPINNVYF AAGALFVSSG FDTSIKLDII 

       370        380        390        400        410 
SDPESERPAI EFETPTFLVS NDDEVSQFCY VSDDESNGEV LEVGKNNFCA LYNLSNP 

References and documentation are available.

Number of amino acids: 417

Molecular weight: 46382.89

Theoretical pI: 5.06

Amino acid composition: 
Ala (A)  22	  5.3%
Arg (R)  16	  3.8%
Asn (N)  32	  7.7%
Asp (D)  30	  7.2%
Cys (C)   6	  1.4%
Gln (Q)  11	  2.6%
Glu (E)  28	  6.7%
Gly (G)  31	  7.4%
His (H)  11	  2.6%
Ile (I)  31	  7.4%
Leu (L)  32	  7.7%
Lys (K)  24	  5.8%
Met (M)   5	  1.2%
Phe (F)  22	  5.3%
Pro (P)  11	  2.6%
Ser (S)  32	  7.7%
Thr (T)  29	  7.0%
Trp (W)   3	  0.7%
Tyr (Y)  12	  2.9%
Val (V)  29	  7.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 40

Atomic composition:

Carbon      C	      2049
Hydrogen    H	      3193
Nitrogen    N	       557
Oxygen      O	       650
Sulfur      S	        11

Formula: C2049H3193N557O650S11
Total number of atoms: 6460

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    34755
Abs 0.1% (=1 g/l)   0.749, assuming all pairs of Cys residues form cystines


Ext. coefficient    34380
Abs 0.1% (=1 g/l)   0.741, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 25.42
This classifies the protein as stable.



Aliphatic index: 84.36

Grand average of hydropathicity (GRAVY): -0.335