ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTKYERDPEL VNFLSKVEDL NSKRYSNIPS SKPAGEALSP VRSHNSGEYR RADMMTGKNV 

        70         80         90        100        110        120 
EGCDNLAYRS AYNYEMTFSP KKTHYSLSEL NLERITPRPD LEGSASQKEK KFLISEEDYL 

       130        140        150        160        170        180 
LLQKLKASQT YNDSNADKNL PSFEKGPRMP SRGRPRPREK EIITIQYDFE LPGRADIPSS 

       190        200        210        220        230        240 
SSSSSPPPLP TRRDHIKITD GNEEKPLLPT RPNKAEVTES PSSRSIKPDA VVPERVKPAP 

       250        260        270        280        290        300 
PVSRSTKPAS FLSSLEDNKL TKAKSYNSEM ETPKTTVKSS HIDYLDSIQL KPTTLSPTMK 

       310        320        330        340        350        360 
NKPKPTPPSP PAKRIPRSES FIKSMLNSNL TTTSKPSLPE KPQKLRNANL AAHKTKPSIP 

       370        380        390        400        410        420 
PKKVELNIVL PELRPVETSP TKQNFENSID LPKLRSSNRN IKKEEEDSIP EAIKGIQNLK 

       430        440        450        460        470        480 
KTKQQKPAIP QKKSFLTNNS KNTTLKNGDD INKLNDEIEA LSLRNNLKKR PPTAPQRKIS 

       490        500        510        520        530        540 
LPEALRKVEL MKKSKTEPVL ESSNELSINA KLDAIIASRN LRASNTLPEL SGVNTNIATS 

       550        560        570 
DKYTTSRDET VKETKPLVHP NKNRTRGPRR KLPTRV 

References and documentation are available.

Number of amino acids: 576

Molecular weight: 64665.62

Theoretical pI: 9.85

Amino acid composition: 
Ala (A)  28	  4.9%
Arg (R)  38	  6.6%
Asn (N)  42	  7.3%
Asp (D)  24	  4.2%
Cys (C)   1	  0.2%
Gln (Q)  12	  2.1%
Glu (E)  45	  7.8%
Gly (G)  13	  2.3%
His (H)   6	  1.0%
Ile (I)  29	  5.0%
Leu (L)  53	  9.2%
Lys (K)  66	 11.5%
Met (M)   9	  1.6%
Phe (F)   9	  1.6%
Pro (P)  61	 10.6%
Ser (S)  67	 11.6%
Thr (T)  41	  7.1%
Trp (W)   0	  0.0%
Tyr (Y)  13	  2.3%
Val (V)  19	  3.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 69
Total number of positively charged residues (Arg + Lys): 104

Atomic composition:

Carbon      C	      2822
Hydrogen    H	      4660
Nitrogen    N	       822
Oxygen      O	       890
Sulfur      S	        10

Formula: C2822H4660N822O890S10
Total number of atoms: 9204

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    19370
Abs 0.1% (=1 g/l)   0.300, assuming all pairs of Cys residues form cystines


Ext. coefficient    19370
Abs 0.1% (=1 g/l)   0.300, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 59.89
This classifies the protein as unstable.



Aliphatic index: 69.95

Grand average of hydropathicity (GRAVY): -0.995