ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTLAFNMQRL VFRNLNVGKR MFKNVPLWRF NVANKLGKPL TRSVGLGGAG IVAGGFYLMN 

        70         80         90        100        110        120 
RQPSKLIFND SLGAAVKQQG PLEPTVGNST AITEERRNKI SSHKQMFLGS LFGVVLGVTV 

       130        140        150        160        170        180 
AKISILFMYV GITSMLLCEW LRYKGWIRIN LKNIKSVIVL KDVDLKKLLI DGLLGTEYMG 

       190 
FKVFFTLSFV LASLNANK 

References and documentation are available.

Number of amino acids: 198

Molecular weight: 22063.35

Theoretical pI: 10.48

Amino acid composition: 
Ala (A)  10	  5.1%
Arg (R)  10	  5.1%
Asn (N)  14	  7.1%
Asp (D)   4	  2.0%
Cys (C)   1	  0.5%
Gln (Q)   5	  2.5%
Glu (E)   5	  2.5%
Gly (G)  19	  9.6%
His (H)   1	  0.5%
Ile (I)  12	  6.1%
Leu (L)  28	 14.1%
Lys (K)  17	  8.6%
Met (M)   8	  4.0%
Phe (F)  13	  6.6%
Pro (P)   5	  2.5%
Ser (S)  12	  6.1%
Thr (T)   9	  4.5%
Trp (W)   3	  1.5%
Tyr (Y)   4	  2.0%
Val (V)  18	  9.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 9
Total number of positively charged residues (Arg + Lys): 27

Atomic composition:

Carbon      C	      1014
Hydrogen    H	      1639
Nitrogen    N	       269
Oxygen      O	       261
Sulfur      S	         9

Formula: C1014H1639N269O261S9
Total number of atoms: 3192

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    22460
Abs 0.1% (=1 g/l)   1.018, assuming all pairs of Cys residues form cystines


Ext. coefficient    22460
Abs 0.1% (=1 g/l)   1.018, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.80
This classifies the protein as stable.



Aliphatic index: 110.20

Grand average of hydropathicity (GRAVY): 0.284