ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTLFSCSVQM PLEERSLTNL PLNLLFRILS HLDMNDLQNI GKTCTLLRML ANENIVYRNA 

        70         80         90        100        110        120 
VIGSNGNMWW TKNVLVDVFD VLNFNRKAMK TLNSHNISLV ASLRNVQRKY KLGVIDPARK 

       130        140        150        160        170        180 
TISYRTNEVE SKEKGSVKDL NMDLNEPTEI TREQIAHTAI LQGMNQFIEL NDKAFRTHSA 

       190        200        210        220        230        240 
DSDDTYIEEN NGEIHSLHGL EKNTTFEEDL VKKPPFIPSP TFSNYSRSST NSVFSSSSPK 

       250        260        270        280        290        300 
LLDDDWNNIT MDFTKSRDPD YKEMTPTSTE SSDSITRLRK SNKVKDKAEL FEKLIFRDSR 

       310        320        330        340        350        360 
PLKTKKKDNP RLKLSSSLSA NDEDFRKIIS PPSDILPKVG RRSVSRGYLE EIERHYPDFN 

       370        380        390        400        410        420 
GETTNPLAIK RVNSTKIANY EQLIIKENSS NCKGITEKND ENKFQRSHTS PVIELSKPHQ 

       430        440        450        460 
RSKLKAVVTD GNKICYRKIE LDNPSGSNTN DHVIKRLDAN TDFNI 

References and documentation are available.

Number of amino acids: 465

Molecular weight: 53168.05

Theoretical pI: 8.85

Amino acid composition: 
Ala (A)  15	  3.2%
Arg (R)  28	  6.0%
Asn (N)  44	  9.5%
Asp (D)  32	  6.9%
Cys (C)   4	  0.9%
Gln (Q)   9	  1.9%
Glu (E)  31	  6.7%
Gly (G)  14	  3.0%
His (H)  10	  2.2%
Ile (I)  31	  6.7%
Leu (L)  43	  9.2%
Lys (K)  41	  8.8%
Met (M)  10	  2.2%
Phe (F)  17	  3.7%
Pro (P)  21	  4.5%
Ser (S)  48	 10.3%
Thr (T)  32	  6.9%
Trp (W)   3	  0.6%
Tyr (Y)  10	  2.2%
Val (V)  22	  4.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 69

Atomic composition:

Carbon      C	      2320
Hydrogen    H	      3736
Nitrogen    N	       666
Oxygen      O	       735
Sulfur      S	        14

Formula: C2320H3736N666O735S14
Total number of atoms: 7471

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    31650
Abs 0.1% (=1 g/l)   0.595, assuming all pairs of Cys residues form cystines


Ext. coefficient    31400
Abs 0.1% (=1 g/l)   0.591, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.32
This classifies the protein as unstable.



Aliphatic index: 79.01

Grand average of hydropathicity (GRAVY): -0.733