ProtParam
User-provided sequence:
10 20 30 40 50 60
MTLINRSKPF VLLVGFSIIS SLLLWVSQAS VVSSGDFNKD FFVTWSPTHV NTSNDGRSRT
70 80 90 100 110 120
LKLDQESGAS FSSIQTFLFG QIDMKIKLIR GSSQGTVVAY YMSSDQPNRD EIDFEFLGNV
130 140 150 160 170 180
NGQPYILQTN VYAEGLDNRE ERIHLWFDPA KDFHTYSILW NIHQIVFMVD QIPIRLYRNH
190 200 210 220 230 240
GEKGVAYPRL QPMSVQASLW NGESWATRGG HDKIDWSKGP FVASFGDYKI DACIWIGNTS
250 260 270 280 290
FCNGESTENW WNKNEFSSLT RVQKRWFKWV RKYHLIYDYC QDYGRFNNKL PKECSLPKY
References and
documentation are available.
Number of amino acids: 299
Molecular weight: 34687.18
Theoretical pI: 8.24
Total number of negatively charged residues (Asp + Glu): 30
Total number of positively charged residues (Arg + Lys): 32
Atomic composition:
Carbon C 1578
Hydrogen H 2360
Nitrogen N 420
Oxygen O 449
Sulfur S 9
Formula: C
1578H
2360N
420O
449S
9
Total number of atoms: 4816
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 85620
Abs 0.1% (=1 g/l) 2.468, assuming all pairs of Cys residues form cystines
Ext. coefficient 85370
Abs 0.1% (=1 g/l) 2.461, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.45
This classifies the protein as unstable.
Aliphatic index: 75.59
Grand average of hydropathicity (GRAVY): -0.412