ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTLKPSKRRK GRSRHSRKKQ ITSAILTEEG IMIKAKPSSP YTYANRMADK RSRSSIDNIS 

        70         80         90        100        110        120 
RTSFQSNISR TSFQSNSDNN SIFETASLIS CVTCLSDTDT IDRSETSTTD TSKDDLSANP 

       130        140        150        160        170        180 
KLHYPSVNGQ LPANTVIPYG RILDARYIEK EPLHYYDANS SPSSPLSSSM SNISEKCDLD 

       190        200        210        220        230        240 
ELESSQKKER KGNSLSRGSN SSSSLLTSRS PFTKLVEVIF ARPRRHDVVP KRVSLYIDYK 

       250        260        270        280        290        300 
PHSSSHLKEE DDLVEEILKR SYKNTRRNKS IFVIINPFGG KGKAKKLFMT KAKPLLLASR 

       310        320        330        340        350        360 
CSIEVVYTKY PGHAIEIARE MDIDKYDTIA CASGDGIPHE VINGLYQRPD HVKAFNNIAI 

       370        380        390        400        410        420 
TEIPCGSGNA MSVSCHWTNN PSYSTLCLIK SIETRIDLMC CSQPSYAREH PKLSFLSQTY 

       430        440        450        460        470        480 
GLIAETDINT EFIRWMGPAR FELGVAFNII QKKKYPCEIY VKYAAKSKNE LKNHYLEHKN 

       490        500        510        520        530        540 
KGSLEFQHIT MNKDNEDCDN YNYENEYETE NEDEDEDADA DDEDSHLISR DLADSSADQI 

       550        560        570        580        590        600 
KEEDFKIKYP LDEGIPSDWE RLDPNISNNL GIFYTGKMPY VAADTKFFPA ALPSDGTMDM 

       610        620        630        640        650        660 
VITDARTSLT RMAPILLGLD KGSHVLQPEV LHSKILAYKI IPKLGNGLFS VDGEKFPLEP 

       670        680 
LQVEIMPRLC KTLLRNGRYV DTDFDSM 

References and documentation are available.

Number of amino acids: 687

Molecular weight: 77565.55

Theoretical pI: 6.42

Amino acid composition: 
Ala (A)  37	  5.4%
Arg (R)  37	  5.4%
Asn (N)  38	  5.5%
Asp (D)  50	  7.3%
Cys (C)  13	  1.9%
Gln (Q)  13	  1.9%
Glu (E)  46	  6.7%
Gly (G)  29	  4.2%
His (H)  17	  2.5%
Ile (I)  52	  7.6%
Leu (L)  55	  8.0%
Lys (K)  54	  7.9%
Met (M)  16	  2.3%
Phe (F)  21	  3.1%
Pro (P)  37	  5.4%
Ser (S)  78	 11.4%
Thr (T)  38	  5.5%
Trp (W)   3	  0.4%
Tyr (Y)  29	  4.2%
Val (V)  24	  3.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 96
Total number of positively charged residues (Arg + Lys): 91

Atomic composition:

Carbon      C	      3399
Hydrogen    H	      5386
Nitrogen    N	       940
Oxygen      O	      1076
Sulfur      S	        29

Formula: C3399H5386N940O1076S29
Total number of atoms: 10830

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    60460
Abs 0.1% (=1 g/l)   0.779, assuming all pairs of Cys residues form cystines


Ext. coefficient    59710
Abs 0.1% (=1 g/l)   0.770, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 57.13
This classifies the protein as unstable.



Aliphatic index: 76.26

Grand average of hydropathicity (GRAVY): -0.603