ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTLLNKFGNC SKHNGLDTVP TPPSPPVEPE RVWREEEEAG PRPAQATPNP AQGHTTPAKR 

        70         80         90        100        110        120 
PAQSKGAAES SIGLKAASSS ACLSILGVME RASLIQKAKL AEQAERYEDM AAFMKSAVEK 

       130        140        150        160        170        180 
GEELSCEERN LLSVAYKNVV GGQRAAWRVL SSIEQKSNEE GSEEKGPEVK EYREKVETEL 

       190        200        210        220        230        240 
RGVCDTVLGL LDSHLIKGAG EAESRVFYLK MKGDYYRYLA EVATGDDKKR IIDSARSAYQ 

       250        260        270        280        290        300 
EAMDISKKEM PPTNPIRLGL ALNFSVFHYE IANSPEEAIS LAKTTFDEAM ADLHTLSEDS 

       310        320        330 
YKDSTLIMQL LRDNLTLWTA DNAGEEGGEA PEEPQS 

References and documentation are available.

Number of amino acids: 336

Molecular weight: 36856.32

Theoretical pI: 4.96

Amino acid composition: 
Ala (A)  37	 11.0%
Arg (R)  17	  5.1%
Asn (N)  12	  3.6%
Asp (D)  15	  4.5%
Cys (C)   4	  1.2%
Gln (Q)  10	  3.0%
Glu (E)  41	 12.2%
Gly (G)  22	  6.5%
His (H)   5	  1.5%
Ile (I)  12	  3.6%
Leu (L)  30	  8.9%
Lys (K)  23	  6.8%
Met (M)   9	  2.7%
Phe (F)   6	  1.8%
Pro (P)  19	  5.7%
Ser (S)  29	  8.6%
Thr (T)  16	  4.8%
Trp (W)   3	  0.9%
Tyr (Y)  10	  3.0%
Val (V)  16	  4.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 56
Total number of positively charged residues (Arg + Lys): 40

Atomic composition:

Carbon      C	      1600
Hydrogen    H	      2553
Nitrogen    N	       445
Oxygen      O	       526
Sulfur      S	        13

Formula: C1600H2553N445O526S13
Total number of atoms: 5137

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    31650
Abs 0.1% (=1 g/l)   0.859, assuming all pairs of Cys residues form cystines


Ext. coefficient    31400
Abs 0.1% (=1 g/l)   0.852, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 55.30
This classifies the protein as unstable.



Aliphatic index: 73.57

Grand average of hydropathicity (GRAVY): -0.592