ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTLMVPFKQV DVFTEKPFMG NPVAVINFLE IDENEVSQEE LQAIANWTNL SETTFLFKPS 

        70         80         90        100        110        120 
DKKYDYKLRI FTPRSELPFA GHPTIGSCKA FLEFTKNTTA TSLVQECKIG AVPITINEGL 

       130        140        150        160        170        180 
ISFKAPMADY ESISSEMIAD YEKAIGLKFI KPPALLHTGP EWIVALVEDA ETCFNANPNF 

       190        200        210        220        230        240 
AMLAHQTKQN DHVGIILAGP KKEAAIKNSY EMRAFAPVIN VYEDPVCGSG SVALARYLQE 

       250        260        270        280        290 
VYKFEKTTDI TISEGGRLKR NGLMLASIKK EADNSTSYYI AGHATTVIDG KIKV 

References and documentation are available.

Number of amino acids: 294

Molecular weight: 32563.44

Theoretical pI: 5.52

Amino acid composition: 
Ala (A)  28	  9.5%
Arg (R)   6	  2.0%
Asn (N)  15	  5.1%
Asp (D)  12	  4.1%
Cys (C)   4	  1.4%
Gln (Q)   7	  2.4%
Glu (E)  24	  8.2%
Gly (G)  16	  5.4%
His (H)   5	  1.7%
Ile (I)  24	  8.2%
Leu (L)  21	  7.1%
Lys (K)  24	  8.2%
Met (M)   8	  2.7%
Phe (F)  16	  5.4%
Pro (P)  16	  5.4%
Ser (S)  17	  5.8%
Thr (T)  21	  7.1%
Trp (W)   2	  0.7%
Tyr (Y)  10	  3.4%
Val (V)  18	  6.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 30

Atomic composition:

Carbon      C	      1472
Hydrogen    H	      2306
Nitrogen    N	       370
Oxygen      O	       437
Sulfur      S	        12

Formula: C1472H2306N370O437S12
Total number of atoms: 4597

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    26150
Abs 0.1% (=1 g/l)   0.803, assuming all pairs of Cys residues form cystines


Ext. coefficient    25900
Abs 0.1% (=1 g/l)   0.795, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 26.91
This classifies the protein as stable.



Aliphatic index: 86.97

Grand average of hydropathicity (GRAVY): -0.105