ProtParam

User-provided sequence:

        10         20         30         40 
MTLPGGVILV FILVGLACIA IIATIIYRKW QARQRGLQRF 

References and documentation are available.

Number of amino acids: 40

Molecular weight: 4500.57

Theoretical pI: 11.56

Amino acid composition: 
Ala (A)   4	 10.0%
Arg (R)   4	 10.0%
Asn (N)   0	  0.0%
Asp (D)   0	  0.0%
Cys (C)   1	  2.5%
Gln (Q)   3	  7.5%
Glu (E)   0	  0.0%
Gly (G)   4	 10.0%
His (H)   0	  0.0%
Ile (I)   7	 17.5%
Leu (L)   5	 12.5%
Lys (K)   1	  2.5%
Met (M)   1	  2.5%
Phe (F)   2	  5.0%
Pro (P)   1	  2.5%
Ser (S)   0	  0.0%
Thr (T)   2	  5.0%
Trp (W)   1	  2.5%
Tyr (Y)   1	  2.5%
Val (V)   3	  7.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 0
Total number of positively charged residues (Arg + Lys): 5

Atomic composition:

Carbon      C	       211
Hydrogen    H	       349
Nitrogen    N	        57
Oxygen      O	        47
Sulfur      S	         2

Formula: C211H349N57O47S2
Total number of atoms: 666

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     6990
Abs 0.1% (=1 g/l)   1.553, assuming all pairs of Cys residues form cystines


Ext. coefficient     6990
Abs 0.1% (=1 g/l)   1.553, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.04
This classifies the protein as unstable.



Aliphatic index: 148.75

Grand average of hydropathicity (GRAVY): 1.028