ProtParam
User-provided sequence:
10 20 30 40 50 60
MTLRSKAIMT STSDKREHLY KILVIGGLGT GKTSFIKRYV HQFFSQNYRA TIGVDFALKV
70 80 90 100 110 120
LQWDANTIVR LQLWDIAGQE RFGNMTRVYY KEAVGAFIVF DVTRSGTFDC VSKWKEDLDS
130 140 150 160 170 180
KVQLPDGSPI PCILLANKCD QEKQGIITQP EKMDEYVREN GFAGWFETSA KENINIDEAA
190 200 210 220
RALVNKILIN DKLISADLAD GDKFNLSAAD ATGSDAKNKC SC
References and
documentation are available.
Number of amino acids: 222
Molecular weight: 24837.33
Theoretical pI: 6.74
Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 29
Atomic composition:
Carbon C 1104
Hydrogen H 1743
Nitrogen N 299
Oxygen O 334
Sulfur S 9
Formula: C
1104H
1743N
299O
334S
9
Total number of atoms: 3489
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 31190
Abs 0.1% (=1 g/l) 1.256, assuming all pairs of Cys residues form cystines
Ext. coefficient 30940
Abs 0.1% (=1 g/l) 1.246, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 32.89
This classifies the protein as stable.
Aliphatic index: 85.27
Grand average of hydropathicity (GRAVY): -0.272