ProtParam
User-provided sequence:
10 20 30 40 50 60
MTLSKYSKPT LNDPNLFRES GYIDGKWVKG TDEVFEVVDP ASGEIIARVP EQPVSVVEEA
70 80 90 100 110 120
IDVAYETFKT YKNTTPRERA KWLRNMYNLM LENLDDLATI ITLENGKALG EAKGEIKYAA
130 140 150 160 170 180
SYFEWYAEEA PRLYGATIQP LNPHNRVFTI RQPVGVCGII CPWNFPSAMI TRKAAAALAV
190 200 210 220 230 240
GCTVVIKPDS QTPLSALAMA YLAEKAGFPK GSFNVILSHA NTPKLGKTLC ESPKVKKVTF
250 260 270 280 290 300
TGSTNVGKIL MKQSSSTLKK LSFELGGNAP FIVFEDADLD QALEQAMACK FRGLGQTCVC
310 320 330 340 350 360
ANRLYVHSSI IDKFAKLLAE RVKKFVIGHG LDPKTTHGCV INSSAIEKVE RHKQDAIDKG
370 380 390 400 410 420
AKVVLEGGRL TELGPNFYAP VILSHVPSTA IVSKEETFGP LCPIFSFDTM EEVVGYANDT
430 440 450 460 470 480
EFGLAAYVFS KNVNTLYTVS EALETGMVSC NTGVFSDCSI PFGGVKESGF GREGSLYGIE
490
DYTVLKTITI GNLPNSI
References and
documentation are available.
Number of amino acids: 497
Molecular weight: 54189.25
Theoretical pI: 6.24
Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 52
Atomic composition:
Carbon C 2437
Hydrogen H 3837
Nitrogen N 629
Oxygen O 725
Sulfur S 20
Formula: C
2437H
3837N
629O
725S
20
Total number of atoms: 7648
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 47955
Abs 0.1% (=1 g/l) 0.885, assuming all pairs of Cys residues form cystines
Ext. coefficient 47330
Abs 0.1% (=1 g/l) 0.873, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 30.28
This classifies the protein as stable.
Aliphatic index: 87.51
Grand average of hydropathicity (GRAVY): -0.053