ProtParam
User-provided sequence:
10 20 30 40 50 60
MTLSNCDSLD NLFQDPPEEE ESSKFVEAVR TLMNRNDMGY PPAAANGTYC LKKIKSLNAK
70 80 90 100 110 120
QWKINKKRMC MLPAVKKKNF DFHEQRSLIL NLNLWKFIKF INCSSKNNYN KNNKHVRSSN
130 140 150 160 170 180
NTVKNENVLP LQKHKKVDND QRLENLFWRS WFKARKRRDI MGKPRERHIK FNDNVEQCII
190 200 210 220 230 240
TDEHFIQRLP STRLNSTDEQ RPCSKSELDP CIGNAASKRS FYDYNSVYVA SDAIITTAAA
250 260 270 280 290 300
TAIISSNSGD YQRGHDVRDV PRNVLLQAGE TDFSSVLRVD SDLKLSNISH HSPVKPSSTS
310 320 330
SHSTFIFESE TDTDTDTDAE TENDIDAYID TSIPNLLL
References and
documentation are available.
Number of amino acids: 338
Molecular weight: 38747.47
Theoretical pI: 8.35
Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 48
Atomic composition:
Carbon C 1686
Hydrogen H 2668
Nitrogen N 490
Oxygen O 533
Sulfur S 13
Formula: C
1686H
2668N
490O
533S
13
Total number of atoms: 5390
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 34295
Abs 0.1% (=1 g/l) 0.885, assuming all pairs of Cys residues form cystines
Ext. coefficient 33920
Abs 0.1% (=1 g/l) 0.875, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.25
This classifies the protein as unstable.
Aliphatic index: 72.13
Grand average of hydropathicity (GRAVY): -0.759