ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTLTIKIGTL NDSDQSAVHN GTENGSDFRK ITPTEEEICD DVVLLWKEEP GTEDATIQHL 

        70         80         90        100        110        120 
YDRITERNQS WKLSASRFRK ILNEHHLYDT DLETVSLYKD KIHFPKALDS DAKVEVKFID 

       130        140        150        160        170        180 
DEHGRGLFAK RDFSKGQIIL KENKPIVYIP PLDKLFLISN GKACARCGKA LYDLTQHKIM 

       190        200        210        220        230        240 
VHYLDCEVCK AIWCSEKCKK AHASLHELLY HSWRSNRIDI LHAGNWKRFV NYCEKYCFTA 

       250        260        270        280        290        300 
AFSVGLIYGS MLLDTTGEVK EQWQKLASIS QRERIKLRDA SGIGSTFSLL NGTTVHTEEE 

       310        320        330        340        350        360 
SDNGTKKGVE KNIDDETVWE KCYELFCGAF PKASEEIDFE KFLTMIGTFN INQYNGQVYH 

       370        380        390        400        410        420 
WISFINHDCE PNAYIEQVEE HEELRLHARK PIKKGEQIRI TYVNPLHGVR LRRRELRVNW 

       430        440        450        460        470        480 
GFLCQCDRCQ NELSTFERVP NLEKKNADAN LGVEKIDSND SSEDGSKKST GNRKSSMREA 

       490        500        510        520 
QPDLKEILKN GKEFELDIPE TVDTQGNVRK TSVRFDSNVS VAVDER 

References and documentation are available.

Number of amino acids: 526

Molecular weight: 60547.35

Theoretical pI: 6.03

Amino acid composition: 
Ala (A)  25	  4.8%
Arg (R)  29	  5.5%
Asn (N)  29	  5.5%
Asp (D)  36	  6.8%
Cys (C)  15	  2.9%
Gln (Q)  16	  3.0%
Glu (E)  49	  9.3%
Gly (G)  29	  5.5%
His (H)  18	  3.4%
Ile (I)  35	  6.7%
Leu (L)  44	  8.4%
Lys (K)  46	  8.7%
Met (M)   5	  1.0%
Phe (F)  21	  4.0%
Pro (P)  13	  2.5%
Ser (S)  35	  6.7%
Thr (T)  29	  5.5%
Trp (W)   9	  1.7%
Tyr (Y)  15	  2.9%
Val (V)  28	  5.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 85
Total number of positively charged residues (Arg + Lys): 75

Atomic composition:

Carbon      C	      2669
Hydrogen    H	      4189
Nitrogen    N	       749
Oxygen      O	       821
Sulfur      S	        20

Formula: C2669H4189N749O821S20
Total number of atoms: 8448

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    72725
Abs 0.1% (=1 g/l)   1.201, assuming all pairs of Cys residues form cystines


Ext. coefficient    71850
Abs 0.1% (=1 g/l)   1.187, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 30.03
This classifies the protein as stable.



Aliphatic index: 78.76

Grand average of hydropathicity (GRAVY): -0.642