ProtParam
User-provided sequence:
10 20 30 40 50 60
MTMAANLARR LIGNRSTQIL GAVNSSSGAA SSVARAFCSS TTPITATLFP GDGIGPEIAE
70 80 90 100 110 120
SVKKVFTTAG VPIEWEEHYV GTEIDPRTQS FLTWESLESV RRNKVGLKGP MATPIGKGHR
130 140 150 160 170 180
SLNLTLRKEL NLYANVRPCY SLPGYKTRYD DVDLITIREN TEGEYSGLEH QVVRGVVESL
190 200 210 220 230 240
KIITRQASLR VAEYAFLYAK THGRERVSAI HKANIMQKTD GLFLKCCREV AEKYPEITYE
250 260 270 280 290 300
EVVIDNCCMM LVKNPALFDV LVMPNLYGDI ISDLCAGLVG GLGLTPSCNI GEDGVALAEA
310 320 330 340 350 360
VHGSAPDIAG KNLANPTALL LSGVMMLRHL KFNEQAEQIH SAIINTIAEG KYRTADLGGS
370
STTTEFTKAI CDHL
References and
documentation are available.
Number of amino acids: 374
Molecular weight: 40624.58
Theoretical pI: 6.33
Total number of negatively charged residues (Asp + Glu): 41
Total number of positively charged residues (Arg + Lys): 38
Atomic composition:
Carbon C 1787
Hydrogen H 2878
Nitrogen N 496
Oxygen O 546
Sulfur S 18
Formula: C
1787H
2878N
496O
546S
18
Total number of atoms: 5725
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 29380
Abs 0.1% (=1 g/l) 0.723, assuming all pairs of Cys residues form cystines
Ext. coefficient 28880
Abs 0.1% (=1 g/l) 0.711, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.55
This classifies the protein as stable.
Aliphatic index: 93.90
Grand average of hydropathicity (GRAVY): -0.058