ProtParam
User-provided sequence:
10 20 30 40 50 60
MTMDGKNKEE EQYLDLCKRI IDEGEFRPDR TGTGTLSLFA PPQLRFSLRD DTFPLLTTKK
70 80 90 100 110 120
VFTRGIILEL LWFLAGDTDA NLLSEQGVKI WDGNGSREYL DKMGFKDRKV GDLGPVYGFQ
130 140 150 160 170 180
WRHFGAKYKT CDDDYTGQGI DQLKQVIHKL KTNPYDRRII MSAWNPADFD KMALPPCHIF
190 200 210 220 230 240
SQFYVSFPKE GEGSGKPRLS CLLYQRSCDM GLGVPFNIAS YALLTRMIAK VVDMEPGEFI
250 260 270 280 290 300
HTLGDAHVYK DHIDALKEQI TRNPRPFPKL KIKRDVKDID DFKLTDFEIE DYNPHPRIQM
KMSV
References and
documentation are available.
Number of amino acids: 304
Molecular weight: 35047.25
Theoretical pI: 6.67
Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 44
Atomic composition:
Carbon C 1578
Hydrogen H 2449
Nitrogen N 421
Oxygen O 453
Sulfur S 15
Formula: C
1578H
2449N
421O
453S
15
Total number of atoms: 4916
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 38640
Abs 0.1% (=1 g/l) 1.103, assuming all pairs of Cys residues form cystines
Ext. coefficient 38390
Abs 0.1% (=1 g/l) 1.095, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.22
This classifies the protein as unstable.
Aliphatic index: 76.97
Grand average of hydropathicity (GRAVY): -0.513