ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTMGDKKSPT RPKRQAKPAA DEGFWDCSVC TFRNSAEAFK CSICDVRKGT STRKPRINSQ 

        70         80         90        100        110        120 
LVAQQVAQQY ATPPPPKKEK KEKVEKPDKE KPEKDKDISP SVTKKNTNKK TKPKSDILKD 

       130        140        150        160        170        180 
PPSEANSIQS ANATTKTSET NHTSRPRLKN VDRSTAQQLA VTVGNVTVII TDFKEKTRSS 

       190        200        210        220 
STSSSTVTSS AGSEQQNQSS SGSESTDKGS SRSSTPKGDM SAVNDESF 

References and documentation are available.

Number of amino acids: 228

Molecular weight: 24776.50

Theoretical pI: 9.66

Amino acid composition: 
Ala (A)  15	  6.6%
Arg (R)  11	  4.8%
Asn (N)  11	  4.8%
Asp (D)  14	  6.1%
Cys (C)   4	  1.8%
Gln (Q)  12	  5.3%
Glu (E)  13	  5.7%
Gly (G)   8	  3.5%
His (H)   1	  0.4%
Ile (I)   7	  3.1%
Leu (L)   4	  1.8%
Lys (K)  30	 13.2%
Met (M)   3	  1.3%
Phe (F)   5	  2.2%
Pro (P)  16	  7.0%
Ser (S)  35	 15.4%
Thr (T)  24	 10.5%
Trp (W)   1	  0.4%
Tyr (Y)   1	  0.4%
Val (V)  13	  5.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 41

Atomic composition:

Carbon      C	      1042
Hydrogen    H	      1727
Nitrogen    N	       317
Oxygen      O	       366
Sulfur      S	         7

Formula: C1042H1727N317O366S7
Total number of atoms: 3459

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     7240
Abs 0.1% (=1 g/l)   0.292, assuming all pairs of Cys residues form cystines


Ext. coefficient     6990
Abs 0.1% (=1 g/l)   0.282, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.59
This classifies the protein as unstable.



Aliphatic index: 41.93

Grand average of hydropathicity (GRAVY): -1.151