ProtParam
User-provided sequence:
10 20 30 40 50 60
MTMTEEKPTS DGKGGWGFFK IPFRNSSGHR NAASSAATSP FPSGASSSST SSHLHNHHQH
70 80 90 100 110 120
HHQHHHQHHH QLGYNGPHGD GSGQNQHPTP SPSVSSVAKS FLPTKRRLKL DPSEKLYFPY
130 140 150 160 170 180
EPGKQVRSAI KIKNTSKSHV AFKFQTTAPK SCFMRPPGAI LAPGETIIAT VFKFVEPPEN
190 200 210 220 230 240
NEKPMDQRSR VKFKIMSLKV KGPMDYVPEL FDEQKDDVSK EQILRVIFLD PERSNPALEK
250 260 270 280 290
LKRQLAEADA AVEARKKPPE ETGPKMIGEG LVIDEWKERR ERYLAQQQGE GADSV
References and
documentation are available.
Number of amino acids: 295
Molecular weight: 32964.13
Theoretical pI: 9.39
Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 42
Atomic composition:
Carbon C 1455
Hydrogen H 2273
Nitrogen N 427
Oxygen O 435
Sulfur S 8
Formula: C
1455H
2273N
427O
435S
8
Total number of atoms: 4598
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 18450
Abs 0.1% (=1 g/l) 0.560, assuming all pairs of Cys residues form cystines
Ext. coefficient 18450
Abs 0.1% (=1 g/l) 0.560, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 45.61
This classifies the protein as unstable.
Aliphatic index: 56.24
Grand average of hydropathicity (GRAVY): -0.882