ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTNEINSATV TQDEVIKWSR EAQSTLEKTQ KICTDAQSSM QKTAQELTIL IPDKLQAIEF 

        70         80         90        100        110        120 
LFKSYREQYD SILKQIETTK IYLHTNIDKV FNDIKDLLDP SLARLNNILL ELKKTRVPSI 

       130        140        150        160        170        180 
VVEGTSEGKT LFDFTSIQSI NLLKENIGIF KSNCSKIKNL LDLEVKEKLN IEQDRMNSRW 

       190        200        210        220        230        240 
NKSVKMYDLI APLQLELRAL IHGASNESNS FMGTILRENQ ALENELVSIL EMQTNHFDQC 

       250        260        270        280        290        300 
MKAVELISSG NGCDMNLGVL KNDAQELPEV FKELTTIYDI ILRNEERSKK FLATHMPNIE 

       310        320        330        340        350        360 
NISDIVKEEL AVFRKFKTEE IPRYTFLIAE CENKLKECSM PVKSDQSPSQ VYTQTLQELT 

       370        380        390        400        410        420 
EHYVKFINIY KTKYLAELHH QQFTYPRKFL KKLTEFLNED IYRIQIEESE RRRQWTSRYG 

       430        440        450        460        470 
EFIPSEFKLP GEHELPVIVQ IITEGLEYIQ KEDGQEEEPN IGNEKELMDM ITGSNK 

References and documentation are available.

Number of amino acids: 476

Molecular weight: 55393.33

Theoretical pI: 5.23

Amino acid composition: 
Ala (A)  16	  3.4%
Arg (R)  18	  3.8%
Asn (N)  30	  6.3%
Asp (D)  21	  4.4%
Cys (C)   6	  1.3%
Gln (Q)  27	  5.7%
Glu (E)  55	 11.6%
Gly (G)  14	  2.9%
His (H)   8	  1.7%
Ile (I)  44	  9.2%
Leu (L)  51	 10.7%
Lys (K)  42	  8.8%
Met (M)  12	  2.5%
Phe (F)  19	  4.0%
Pro (P)  14	  2.9%
Ser (S)  32	  6.7%
Thr (T)  31	  6.5%
Trp (W)   3	  0.6%
Tyr (Y)  14	  2.9%
Val (V)  19	  4.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 76
Total number of positively charged residues (Arg + Lys): 60

Atomic composition:

Carbon      C	      2461
Hydrogen    H	      3942
Nitrogen    N	       648
Oxygen      O	       763
Sulfur      S	        18

Formula: C2461H3942N648O763S18
Total number of atoms: 7832

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    37735
Abs 0.1% (=1 g/l)   0.681, assuming all pairs of Cys residues form cystines


Ext. coefficient    37360
Abs 0.1% (=1 g/l)   0.674, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 56.37
This classifies the protein as unstable.



Aliphatic index: 92.77

Grand average of hydropathicity (GRAVY): -0.506