ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTNIKSEQKE SEELVLPNDR PQYEQRVTKQ PTSGKYIIIT ITLISIIIVG FGLGLCAQIY 

        70         80         90        100        110        120 
FQAESDVLPI CDKRIIGYYS GSGTSNITST QLSNLTHTVF AFVYMTPDGT LLFNNQIEKN 

       130        140        150        160        170        180 
RFLKLKEVVR NGNSKIKLMF SIGGKDNSKY FSSVIPSEER IQTFIESILE FLEAYDLDGV 

       190        200        210        220        230        240 
DLFWKWPEEE YRDAYFAFIN QLRKTFEAKK KYYILSIVIP PPTLDGWGKK RLNKIIESVD 

       250        260        270        280        290        300 
FINAYSTDYY TPLADNLSDD IAGPSAPIYF GAQGKSERNV HHTARNYSCL TKQASKFNIV 

       310        320        330        340        350        360 
IPFYATLWEN VYETVLDSKR GVYRHVAPFY GRTVGKTYLS RLAVKQEGFQ VESYSYDPAP 

       370        380        390        400        410        420 
RAAFSYNRTI GIYLTFETKE SIRAKIDYVK DRILGGVWIF SVDMDDETNS QLKAVSFDGF 

       430 
CTIGNGSKYE CV 

References and documentation are available.

Number of amino acids: 432

Molecular weight: 49311.11

Theoretical pI: 6.91

Amino acid composition: 
Ala (A)  22	  5.1%
Arg (R)  19	  4.4%
Asn (N)  21	  4.9%
Asp (D)  23	  5.3%
Cys (C)   5	  1.2%
Gln (Q)  15	  3.5%
Glu (E)  27	  6.2%
Gly (G)  27	  6.2%
His (H)   4	  0.9%
Ile (I)  39	  9.0%
Leu (L)  31	  7.2%
Lys (K)  31	  7.2%
Met (M)   4	  0.9%
Phe (F)  25	  5.8%
Pro (P)  17	  3.9%
Ser (S)  35	  8.1%
Thr (T)  28	  6.5%
Trp (W)   5	  1.2%
Tyr (Y)  28	  6.5%
Val (V)  26	  6.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 50
Total number of positively charged residues (Arg + Lys): 50

Atomic composition:

Carbon      C	      2249
Hydrogen    H	      3453
Nitrogen    N	       569
Oxygen      O	       660
Sulfur      S	         9

Formula: C2249H3453N569O660S9
Total number of atoms: 6940

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    69470
Abs 0.1% (=1 g/l)   1.409, assuming all pairs of Cys residues form cystines


Ext. coefficient    69220
Abs 0.1% (=1 g/l)   1.404, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.58
This classifies the protein as stable.



Aliphatic index: 85.74

Grand average of hydropathicity (GRAVY): -0.265