ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTNINRLSMS TMFNALHEIL NEEPLAIPPP PSRGGYSYFH IRTTEMDLQR MFNFMTGSED 

        70         80         90        100        110        120 
LKDDISVLDT GMLKLMRMSD DMDVFFFVAI MRLFIHVMID KYASYVAIQG MRIFKQDKRE 

       130        140        150        160        170        180 
LMYDHILRYA LFLARDQYGC IALNEIIKEL DDPYYRDELM DIVSNNALLL SNDAYGNFVV 

       190        200        210        220        230        240 
QHVLKLHDSR CTGNIADKLC GYCVELSFKK YGSYIVERLL EVRDIPMATI VLDLLACKTE 

       250        260        270        280        290        300 
MLIRLARSEN GNFVVCKLLE LTNDILTADL FYSLVNKLRP YRFLLHRFPE SKIVAILGSM 


RVPN 

References and documentation are available.

Number of amino acids: 304

Molecular weight: 35270.28

Theoretical pI: 5.84

Amino acid composition: 
Ala (A)  16	  5.3%
Arg (R)  20	  6.6%
Asn (N)  16	  5.3%
Asp (D)  23	  7.6%
Cys (C)   6	  2.0%
Gln (Q)   5	  1.6%
Glu (E)  16	  5.3%
Gly (G)  12	  3.9%
His (H)   7	  2.3%
Ile (I)  23	  7.6%
Leu (L)  41	 13.5%
Lys (K)  14	  4.6%
Met (M)  18	  5.9%
Phe (F)  16	  5.3%
Pro (P)  10	  3.3%
Ser (S)  17	  5.6%
Thr (T)  11	  3.6%
Trp (W)   0	  0.0%
Tyr (Y)  15	  4.9%
Val (V)  18	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 34

Atomic composition:

Carbon      C	      1585
Hydrogen    H	      2507
Nitrogen    N	       413
Oxygen      O	       447
Sulfur      S	        24

Formula: C1585H2507N413O447S24
Total number of atoms: 4976

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    22725
Abs 0.1% (=1 g/l)   0.644, assuming all pairs of Cys residues form cystines


Ext. coefficient    22350
Abs 0.1% (=1 g/l)   0.634, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.65
This classifies the protein as stable.



Aliphatic index: 104.54

Grand average of hydropathicity (GRAVY): 0.063