ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTNMAEDKTS ISKVHSEFSN SSNTTGSEEE SRSHQESFDG ESSSGESKSK SKLNLEYSAD 

        70         80         90        100        110        120 
IEPLKFRMTK TNNTNDKLKY SNNGNATDSF MRLKEHLQRG NTLNSNLRVN EFYPFNSIDT 

       130        140        150        160        170        180 
EQFENYLREP KYIKMLKRRK NLKQFRRLFL AQELMAYEGE TVTSTSKSSE PTSKAIWSTK 

       190        200        210        220        230        240 
FSRDGKFMAT GSKDGKIRIW KVIGSPVERA ELDSSAESNK EARAKSMRIK QQVSSLNNPK 

       250        260        270        280        290        300 
EKQFLDSATE KYEEKEKLLN LYAPVFHPTP LRLYKEHVQD VLDINWSKNN FILSASMDKT 

       310        320        330        340        350        360 
VKLWHPDRKN SLKTFIHPDF VTCVEFHPTD DRFFISGCLD HKCRLWSILD DEVSFEYDCQ 

       370        380        390        400        410        420 
DLITSVTLSP EEGKYTIIGT FNGYVHILMT RGLTPVSSFH VADRQTQEQN AHVMVTETDS 

       430        440        450        460        470        480 
KIRHGPRVTG LQAFRSQLDN SFRLVVTSND SRIRIFDLEQ RKLLEVLKGF HSGSSQHKAQ 

       490        500        510        520        530        540 
LSIWHGQPIV VNSSDDHWVY GWRLKSSDRE NDQDEPKRKP KGLARSGSLR SIFSKSMSRS 

       550        560        570        580        590        600 
SSQNNEEKPH HHLKLTNLLP LPHHSNDHYI KNTDYISFHA HNAPVTCVSI APPETSKTLS 

       610        620        630        640        650        660 
LSNDVICELS LEFFQTSDSF DVLSRSNDDG IMSDVESSLG YNSKPGSISN ASATSAIPDV 

       670        680        690        700        710        720 
VDAIGTILIS TDNVGTIRVF RADMPSVIRK RVLLKLEEYN REVRRRFNSS DSLHSLSRSF 

       730        740        750        760        770        780 
NSRAKSNLAG QPAAAYTNTG KGYATGRGYS NICPKSSTSL KTLGSNAQPR TPRESMSSIF 

       790        800        810        820        830 
SNAHGPTTPT SAMNLPIRCN VCNGSRFEAF SGANDQQDRN YYCVDCGTVV NNFR 

References and documentation are available.

Number of amino acids: 834

Molecular weight: 94254.36

Theoretical pI: 8.86

Amino acid composition: 
Ala (A)  38	  4.6%
Arg (R)  52	  6.2%
Asn (N)  57	  6.8%
Asp (D)  48	  5.8%
Cys (C)  11	  1.3%
Gln (Q)  26	  3.1%
Glu (E)  51	  6.1%
Gly (G)  38	  4.6%
His (H)  27	  3.2%
Ile (I)  39	  4.7%
Leu (L)  65	  7.8%
Lys (K)  58	  7.0%
Met (M)  16	  1.9%
Phe (F)  38	  4.6%
Pro (P)  34	  4.1%
Ser (S) 111	 13.3%
Thr (T)  53	  6.4%
Trp (W)   8	  1.0%
Tyr (Y)  22	  2.6%
Val (V)  42	  5.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 99
Total number of positively charged residues (Arg + Lys): 110

Atomic composition:

Carbon      C	      4107
Hydrogen    H	      6467
Nitrogen    N	      1193
Oxygen      O	      1302
Sulfur      S	        27

Formula: C4107H6467N1193O1302S27
Total number of atoms: 13096

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    77405
Abs 0.1% (=1 g/l)   0.821, assuming all pairs of Cys residues form cystines


Ext. coefficient    76780
Abs 0.1% (=1 g/l)   0.815, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.23
This classifies the protein as unstable.



Aliphatic index: 67.79

Grand average of hydropathicity (GRAVY): -0.699