ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTNMKQRLRN LFRRNKMRAR PTTVASDANK EQPEPPKQEP SKQKKTKQDA SKQEPPKQEE 

        70         80         90        100        110        120 
PEKPPKEVEV EIIPSPTSEG EMPTYFHVGA LAKDCLINGF KIGAWQMHCS TRTDNDFYLN 

       130        140        150        160        170        180 
TFGEGYPTMK NVFGGMEVFK EVGNYSTSLG WFTNNDLLSE IAVRGMNFGS RLYGLLKSTI 

       190        200        210        220        230        240 
GTKDEVSFQT KLKCGLERDP VKVELVVPLY NEPLFLGYVL VAPVENWVLG YRTEYNFDEK 

       250        260        270        280        290        300 
GFDKHALCLG YNNGRTEVGL KLENFEDLRG SIFQRIGEAW AFAIKTNLYS SENVKQFAIG 

       310        320        330        340        350        360 
VQYDFQNGTM VKAKLREDSR MGFVYQSKIG ENIDVGYHLA FDGVDPIGGA HRIGVSWGFH 


C 

References and documentation are available.

Number of amino acids: 361

Molecular weight: 40908.48

Theoretical pI: 7.02

Amino acid composition: 
Ala (A)  17	  4.7%
Arg (R)  17	  4.7%
Asn (N)  22	  6.1%
Asp (D)  16	  4.4%
Cys (C)   5	  1.4%
Gln (Q)  14	  3.9%
Glu (E)  30	  8.3%
Gly (G)  33	  9.1%
His (H)   6	  1.7%
Ile (I)  14	  3.9%
Leu (L)  27	  7.5%
Lys (K)  29	  8.0%
Met (M)  10	  2.8%
Phe (F)  21	  5.8%
Pro (P)  19	  5.3%
Ser (S)  18	  5.0%
Thr (T)  19	  5.3%
Trp (W)   5	  1.4%
Tyr (Y)  14	  3.9%
Val (V)  25	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 46
Total number of positively charged residues (Arg + Lys): 46

Atomic composition:

Carbon      C	      1832
Hydrogen    H	      2826
Nitrogen    N	       494
Oxygen      O	       541
Sulfur      S	        15

Formula: C1832H2826N494O541S15
Total number of atoms: 5708

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    48610
Abs 0.1% (=1 g/l)   1.188, assuming all pairs of Cys residues form cystines


Ext. coefficient    48360
Abs 0.1% (=1 g/l)   1.182, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.90
This classifies the protein as stable.



Aliphatic index: 69.09

Grand average of hydropathicity (GRAVY): -0.549