ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTNSNFNHGN HGSNLPARLF TKQSQALIYN YKEAAVQRML DFDNVSQRDT PSVGGLIHPG 

        70         80         90        100        110        120 
SDGGMYKAFF GFKELVIPVY NSVSEACQQC PNADVFLNFA SHRSAYQSSL LALREPSIQT 

       130        140        150        160        170        180 
VVIIAEGVPE NEARSLISIA KKLGKVIIGP ATVGGIQAGC FKIGNTAGTI VYIMACKLYR 

       190        200        210        220        230        240 
SGSVGFVSKS GGLSNEMYNV LSRCTDGIYE GIAIGGDAFP GSTLTDHALR YEKLPEVQMI 

       250        260        270        280        290        300 
VILGELGGWD EYGIVEALKK GEITKPICAW VSGTVAKIFP TEVQFGHAGA KSGGETESAD 

       310        320        330        340        350        360 
AKNKALREAG AVVPTSFEDF SNVIAATYAK LQSKGLVKPV EEPTPPELPL DFKTAVKAGK 

       370        380        390        400        410        420 
VRKPTSIIST ICDDRGDELS YAGVPISEVC KEQYNMGDVI GLLWFKRKLP PYASKFFEMC 

       430        440        450        460        470        480 
LKLVADHGPC VSGAHNTIVA ARAGKDLVSS LVSGLLTIGP RFGGAIDDSA RVFQDAVDNN 

       490        500        510        520        530        540 
LQPSQFVEGM KSKGKRIPGI GHLIKSADEI DKRVVLLKDY AFTHFSSTKY LEYALEVEKY 

       550        560        570        580        590        600 
TLQKANNLIL NVDGCIGVLF LDLLHSSGLF TQHEIKEIID VGYLNGFFIV GRSVGLIGHA 

       610        620 
LDQRRNKQGL YRHQADDVHY AL 

References and documentation are available.

Number of amino acids: 622

Molecular weight: 67320.10

Theoretical pI: 6.94

Amino acid composition: 
Ala (A)  52	  8.4%
Arg (R)  22	  3.5%
Asn (N)  25	  4.0%
Asp (D)  31	  5.0%
Cys (C)  11	  1.8%
Gln (Q)  21	  3.4%
Glu (E)  34	  5.5%
Gly (G)  62	 10.0%
His (H)  15	  2.4%
Ile (I)  43	  6.9%
Leu (L)  55	  8.8%
Lys (K)  42	  6.8%
Met (M)   9	  1.4%
Phe (F)  28	  4.5%
Pro (P)  26	  4.2%
Ser (S)  46	  7.4%
Thr (T)  26	  4.2%
Trp (W)   3	  0.5%
Tyr (Y)  22	  3.5%
Val (V)  49	  7.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 64

Atomic composition:

Carbon      C	      3019
Hydrogen    H	      4761
Nitrogen    N	       809
Oxygen      O	       893
Sulfur      S	        20

Formula: C3019H4761N809O893S20
Total number of atoms: 9502

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    49905
Abs 0.1% (=1 g/l)   0.741, assuming all pairs of Cys residues form cystines


Ext. coefficient    49280
Abs 0.1% (=1 g/l)   0.732, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.77
This classifies the protein as stable.



Aliphatic index: 92.65

Grand average of hydropathicity (GRAVY): -0.044