ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTNTFEIVFA VPMECDSCVD SIASVLKGLD GVEKFNINLK DNLVSTEGSL PPSEISKAIQ 

        70         80         90        100        110        120 
STGKDAIIRG TGKPDSAAVC ILESFDPNDI QHPVKGLARL VEVSPNDLFV DLTVNGLPKG 

       130        140        150        160        170        180 
VYYPSIRVSG NLSQGALSTG PSFYELNPIE VKTPVNAETT ISSRGAKLEE DSTLYAGQAF 

       190        200        210        220        230        240 
LHAKLNINQL IGRSIILSKI KDQVAPDSLC GVIARSAGVW ENDKQVCSCS GKTVWQERID 


ARVKGVHV 

References and documentation are available.

Number of amino acids: 248

Molecular weight: 26559.27

Theoretical pI: 5.30

Amino acid composition: 
Ala (A)  17	  6.9%
Arg (R)   8	  3.2%
Asn (N)  13	  5.2%
Asp (D)  15	  6.0%
Cys (C)   6	  2.4%
Gln (Q)   8	  3.2%
Glu (E)  14	  5.6%
Gly (G)  19	  7.7%
His (H)   3	  1.2%
Ile (I)  19	  7.7%
Leu (L)  21	  8.5%
Lys (K)  16	  6.5%
Met (M)   2	  0.8%
Phe (F)   7	  2.8%
Pro (P)  13	  5.2%
Ser (S)  25	 10.1%
Thr (T)  12	  4.8%
Trp (W)   2	  0.8%
Tyr (Y)   4	  1.6%
Val (V)  24	  9.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 24

Atomic composition:

Carbon      C	      1170
Hydrogen    H	      1891
Nitrogen    N	       317
Oxygen      O	       369
Sulfur      S	         8

Formula: C1170H1891N317O369S8
Total number of atoms: 3755

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    17335
Abs 0.1% (=1 g/l)   0.653, assuming all pairs of Cys residues form cystines


Ext. coefficient    16960
Abs 0.1% (=1 g/l)   0.639, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.32
This classifies the protein as unstable.



Aliphatic index: 97.82

Grand average of hydropathicity (GRAVY): -0.047