ProtParam
User-provided sequence:
10 20 30 40 50 60
MTNWQQQLPL TDTQKNELDK SVLRYLNWNY KQTVRHEHAQ DYESVRHAIV TLSGFLLQES
70 80 90 100 110 120
VDRQEFISNN DTSNESMVDI DELLLPKKWN SIVRLQKKII ELEQNTETLV SQIKDLNTQV
130 140 150 160 170 180
SELAQFKPTT SNGTSAHNVL KWIPRNLPSC LINVESSVTS VKLHPNLPIV FVATDHGKLY
190 200 210 220 230 240
AFDLFNYTIP LASLQSHTKA ITSMDVLFTN YTNSSKKNYL VIVTASKDLQ IHVFKWVSEE
250 260 270 280 290 300
CKFQQIRSLL GHEHIVSAVK IWQKNNDVHI ASCSRDQTVK IWDFHNGWSL KTFQPHSQWV
310 320 330 340 350 360
RSIDVLGDYI ISGSHDTTLR LTHWPSGNGL SVGTGHEFPI EKVKFIHFIE DSPEIRFRTP
370 380 390 400 410 420
STDRYKNWGM QYCVSASRDR TIKIWEIPLP TLMAHRAPIP NPTDSNFRCV LTLKGHLSWV
430 440 450 460 470 480
RDISIRGQYL FSCADDKSVR CWDLNTGQCL HVWEKLHTGF VNCLDLDVDF DSNVTPRQMM
490
VTGGLDCKSN VFMR
References and
documentation are available.
Number of amino acids: 494
Molecular weight: 56900.74
Theoretical pI: 7.32
Total number of negatively charged residues (Asp + Glu): 52
Total number of positively charged residues (Arg + Lys): 52
Atomic composition:
Carbon C 2545
Hydrogen H 3956
Nitrogen N 702
Oxygen O 746
Sulfur S 18
Formula: C
2545H
3956N
702O
746S
18
Total number of atoms: 7967
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 99515
Abs 0.1% (=1 g/l) 1.749, assuming all pairs of Cys residues form cystines
Ext. coefficient 98890
Abs 0.1% (=1 g/l) 1.738, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 28.90
This classifies the protein as stable.
Aliphatic index: 87.71
Grand average of hydropathicity (GRAVY): -0.362