ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTPEAKKRKN QKKKLKQKQK KAAEKAASHS EEPLELPEST INSSFNDDSV NRTESDIASK 

        70         80         90        100        110        120 
SDVPPVSSST NISPANETQL EIPDTQELHH KLLNDSDQHD ITADSNDLPD NSIVEHDSVI 

       130        140        150        160        170        180 
TQTKPAMSQE YEETAAHLSS RNPSLDVVAG ELHNNNEHTQ KIAVSAVEED SFNEEEGENH 

       190        200        210        220        230        240 
DSIIISSLND ATPSQYNHFL PSDGNLLSPE LSSGDTPTHN VPLGTKDNEI NDDEYCNDKE 

       250        260        270        280        290        300 
ISLNANNVLP DELSKEEDER LKLETHVSTE EKKQDIADQE TAENLFTSST EPSENKIRNS 

       310        320        330        340        350        360 
GDDTSMLFQD DESDQKVPWE EDVKKDFHNE NTNNTQESAP NTDDRDKGYE GNEALKKSES 

       370        380        390        400        410        420 
CTAADERSYS EETSEDIFHG HDKQVVEGQN DFTGKNIENE SQKLMGEGNH KLPLSAEADI 

       430        440        450        460        470        480 
IEPGKDIQDQ AEDLFTQSSG DLGEVLPWES TDKNADVTSK SQEKHEDLFA ASGNDEKLPW 

       490        500        510        520        530        540 
EVSDGEVSSG KTENSMQTST EKIAEQKFSF LENDDDLLDD DDSFLASSEE EDTVPNTDNT 

       550        560        570        580        590        600 
TNLTSKPVEE KKASRYKPII EEEAGMRQEQ VHFTNTTGIV TPQQFHGLTK TGLGTPNQQV 

       610        620        630        640        650        660 
SVPNIVSPKP PVVKDNRSNF KINEEKKKSD AYDFPLEIIS ESSKKGHAKP VAVPTQRFGS 

       670        680        690        700        710        720 
GNSFSSLDKP IPQSRKGSNN SNRPPVIPLG TQEPRSSRTN SAISQSPVNY AFPNPYKIQQ 

       730        740        750        760        770        780 
LQQAPIQSGM PLPNTNIPPP ALKVETTVSA PPIRARGVSN ASVGSSASFG ARHATQYGLN 

       790        800        810        820        830        840 
NGVPPVSPYG QATINLPTAN KYAPVSPTVQ QKQYPSVVQN LGASAVNTPN FVKTHRGHTS 

       850        860        870        880        890        900 
SISSYTPNQN EHASRYAPNY QQSYQVPYTS QPVGPVAGNS SYQSQTRSSY AVPMMPQAQT 

       910        920        930        940        950        960 
SASIQPHANI QPPTGILPLA PLRPLDPLQA ATNLQPRASN ITAANSLPLA NLPLAENILP 

       970        980        990       1000       1010       1020 
EIITHRATSS VAPPRQENNP IKIDNEALLR RQFPIFHWSA ANKVVYAVPP IPDQSQYMIS 

      1030       1040       1050       1060       1070       1080 
SSIVQEIKVT PIDQIIKPND MLKSFPGPLG SAKLKKKDLT KWMETTIKSI SENESSTDMT 

      1090       1100       1110       1120       1130       1140 
IWQLLEMKLN DKVNWKNISK LLYNSDELLM YLSQPFPNGD MIPNAYRLDI NCQMRVLAFL 

      1150       1160       1170       1180       1190       1200 
QTGNHDEALR LALSKRDYAI ALLVGSLMGK DRWSEVIQKY LYEGFTAGPN DQKELAHFLL 

      1210       1220       1230       1240       1250       1260 
LIFQVFVGNS KMAIKSFYTN NETSQWASEN WKSIVAAVLI NIPENNEDPL LIPPVVLEFL 

      1270       1280       1290       1300       1310       1320 
IEFGIFLTKK GLTAAASTLF IIGNVPLSNE PVMADSDVIF ESIGNMNTFE SILWDEIYEY 

      1330       1340       1350       1360       1370       1380 
IFSYDPKFKG FSSILPQKIY HASLLQEQGL NSLGTKYTDY LSSSVRKLPK KDILTINLTR 

      1390       1400       1410       1420       1430       1440 
ELSEVASRLS ESNTGWLAKP KLSSVWGQLD KSFNKYIGGD DIDALNKKND KKKVFDGFTP 

      1450       1460       1470       1480       1490       1500 
GSSANSSTVD LTQTFTPFQA QVTSQSYVDT TALLHNAHNV PSHSVLHSKP SNVSKGLVEA 

      1510       1520       1530       1540       1550       1560 
NLPYTHRIGD SLQGSPQRIH NTQFAAAEPQ MASLRRVRTD QHTNEKALKS QQILEKKSTA 

      1570       1580       1590       1600       1610       1620 
YTPQFGQNHS VPMEKSNSNV PSLFADFPAP PKLGTVPSNY VSSPDLVRRE SIISTGSEFL 

      1630       1640       1650       1660       1670       1680 
PPPKIGVPTK ANSSQGSLMY SPSVEALPID PVVPQVHETG YNDFGNKHSQ KSMPEDESHT 

      1690       1700       1710       1720       1730       1740 
SHDNSNADQN TLKDSADVTD ETMDIEGPGF NDVKNLLPME PNHQPTSTVN PIQTISDDIQ 

      1750       1760       1770       1780       1790       1800 
PILQTNVEVR GTDASKMENS LPSIENERSS EEQPENISKS ASSAYLPSTG GLSLENRPLT 

      1810       1820       1830       1840       1850       1860 
QDENSISETV QSTYLPAGSI SMEAKPISQV QDVPRNVNNK ASKLVEQHMA PPKPKSTDAT 

      1870       1880       1890       1900       1910       1920 
KMNYSPYVPQ STAASADGDE STILKTSPAI YARTHQAHAS NPSQYFPLVN QANETASFEL 

      1930       1940       1950       1960       1970       1980 
SESTSQAQSN GNVASENRFS PIKKAEVVEK DTFQPTIRKA STNQYRAFKP LESDADKYND 

      1990       2000       2010       2020       2030       2040 
VIEDESDDDN MSTDEAKNRK EEKKNVNMKK ETKPSNKDID DKSNGWFGWL KKDTGDKKVY 

      2050       2060       2070       2080       2090       2100 
KAKLGHKNTL YYDEKLKRWV NKDATEEEKQ KIIESSAPPP PPIVKRKDGG PKTKPRSGPI 

      2110       2120       2130       2140       2150       2160 
NNSLPPVHAT SVIPNNPITG EPLPIKTSPS PTGPNPNNSP SPSSPISRIS GVNLTSKKAN 

      2170       2180       2190 
GLDDLLSLAG GPKPASTRRK KKTARGYVNV MDNIQ 

References and documentation are available.

Number of amino acids: 2195

Molecular weight: 241695.79

Theoretical pI: 5.21

Amino acid composition: 
Ala (A) 141	  6.4%
Arg (R)  58	  2.6%
Asn (N) 168	  7.7%
Asp (D) 135	  6.2%
Cys (C)   3	  0.1%
Gln (Q) 113	  5.1%
Glu (E) 158	  7.2%
Gly (G)  95	  4.3%
His (H)  48	  2.2%
Ile (I) 116	  5.3%
Leu (L) 155	  7.1%
Lys (K) 162	  7.4%
Met (M)  34	  1.5%
Phe (F)  59	  2.7%
Pro (P) 175	  8.0%
Ser (S) 249	 11.3%
Thr (T) 141	  6.4%
Trp (W)  16	  0.7%
Tyr (Y)  54	  2.5%
Val (V) 115	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 293
Total number of positively charged residues (Arg + Lys): 220

Atomic composition:

Carbon      C	     10547
Hydrogen    H	     16632
Nitrogen    N	      2924
Oxygen      O	      3507
Sulfur      S	        37

Formula: C10547H16632N2924O3507S37
Total number of atoms: 33647

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   168585
Abs 0.1% (=1 g/l)   0.698, assuming all pairs of Cys residues form cystines


Ext. coefficient   168460
Abs 0.1% (=1 g/l)   0.697, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.07
This classifies the protein as unstable.



Aliphatic index: 69.77

Grand average of hydropathicity (GRAVY): -0.761