ProtParam
User-provided sequence:
10 20 30 40 50 60
MTPEQKAKLE ANRKLAIERL RKRGILSSDQ LNRIESRNEP LKTRPLAVTS GSNRDDNAAA
70 80 90 100 110 120
AVHVPNHNGQ PSALANTNTN TTSLYGSGVV DGSKRDASVL DKRPTDRIRP SIRKQDYIEY
130 140 150 160 170 180
DFATMQNLNG GYINPKDKLP NSDFTDDQEF ESEFGSKKQK TLQDWKKEQL ERKMLYENAP
190 200 210 220 230 240
PPEHISKAPK CIECHINIEM DPVLHDVFKL QVCKQCSKEH PEKYALLTKT ECKEDYFLTD
250 260 270 280 290 300
PELNDEDLFH RLEKPNPHSG TFARMQLFVR CEVEAFAFKK WGGEEGLDEE WQRREEGKAH
310 320 330 340 350 360
RREKKYEKKI KEMRLKTRAQ EYTNRLREKK HGKAHIHHFS DPVDGGIDED GYQIQRRRCT
370
DCGLETEEID I
References and
documentation are available.
Number of amino acids: 371
Molecular weight: 43038.31
Theoretical pI: 6.91
Total number of negatively charged residues (Asp + Glu): 66
Total number of positively charged residues (Arg + Lys): 65
Atomic composition:
Carbon C 1868
Hydrogen H 2966
Nitrogen N 556
Oxygen O 586
Sulfur S 14
Formula: C
1868H
2966N
556O
586S
14
Total number of atoms: 5990
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 31900
Abs 0.1% (=1 g/l) 0.741, assuming all pairs of Cys residues form cystines
Ext. coefficient 31400
Abs 0.1% (=1 g/l) 0.730, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 51.59
This classifies the protein as unstable.
Aliphatic index: 62.35
Grand average of hydropathicity (GRAVY): -1.107