ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTPISQRISQ KSPKLRETKQ IQMTTTDKSA GIVTDSEAGP ETSLLLNPHE GSTKKAPSDS 

        70         80         90        100        110        120 
YHFAYIIYFT LGVGFLLPWN AFITAVDYFS YLYPSTAVDR IFAVIYMLVA LVCLFVIVVF 

       130        140        150        160        170        180 
YAHKSLASFR INLGLLLFVI ALLVVPVLDL VYVKGQVGLY AGFDVTSAAV ALSGLGDALM 

       190        200        210        220        230        240 
QGGLIGVAGE MPERYMQAVV AGTAGSGVLV SLLRILTKAV YPQDPDGLRK SANLYFAVGI 

       250        260        270        280        290        300 
VVMVICAVFY NVAHKLPVIK FHEERKNEEL IREKSEEKGS LTGLAWRTTL WDIVTKVKSH 

       310        320        330        340        350        360 
GFGIVLLYMV TLSIFPGYIT EDVHSELLTD WYPILLIAAY NVFDLVGKCL TAVFMLEDEK 

       370        380        390        400        410        420 
IAVGGSIARL LFYPLFWGCL HGPMFLRTEI PVTILTCLLG LTNGYLTSVL MILAPKSVPL 

       430        440        450 
RHSETAGIVT VMFLVVGLAS GSVIAWFWVI 

References and documentation are available.

Number of amino acids: 450

Molecular weight: 49344.32

Theoretical pI: 6.76

Amino acid composition: 
Ala (A)  36	  8.0%
Arg (R)  13	  2.9%
Asn (N)   8	  1.8%
Asp (D)  15	  3.3%
Cys (C)   5	  1.1%
Gln (Q)   8	  1.8%
Glu (E)  19	  4.2%
Gly (G)  35	  7.8%
His (H)   9	  2.0%
Ile (I)  32	  7.1%
Leu (L)  61	 13.6%
Lys (K)  20	  4.4%
Met (M)  12	  2.7%
Phe (F)  23	  5.1%
Pro (P)  19	  4.2%
Ser (S)  29	  6.4%
Thr (T)  29	  6.4%
Trp (W)   7	  1.6%
Tyr (Y)  20	  4.4%
Val (V)  50	 11.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 33

Atomic composition:

Carbon      C	      2302
Hydrogen    H	      3610
Nitrogen    N	       550
Oxygen      O	       613
Sulfur      S	        17

Formula: C2302H3610N550O613S17
Total number of atoms: 7092

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    68550
Abs 0.1% (=1 g/l)   1.389, assuming all pairs of Cys residues form cystines


Ext. coefficient    68300
Abs 0.1% (=1 g/l)   1.384, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.88
This classifies the protein as stable.



Aliphatic index: 120.82

Grand average of hydropathicity (GRAVY): 0.644